Differential miRNA expression profiling of urinary exosomes from normo- and microalbuminuric type 1 diabetic patients

ABSTRACT: The aim of this study was to compare miRNA expression in urinary exosomes from type 1 diabetic patients with and without incipient diabetic nephropathy. Overnight urine collections were obtained from normo- and microalbuminuric type 1 diabetic patients. Urines were pre-cleared by both centrifugation and filtration, urinary exosomes were isolated by two consecutive ultracentrifugation steps and total RNA extracted. Differential miRNA profiling was performed using a Human TaqMan miRNA Array A on an 7900HT Fast Real-Time PCR System. Results showed that expression of 22 urinary exosomal miRNAs differed in incipient diabetic nephropathy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE48318 | GEO | 2014/02/14

SECONDARY ACCESSION(S): PRJNA209697

REPOSITORIES: GEO