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Transcriptomic comparison of tumoric tissues induced by different c58 agrobacterium lines; Quorum-sensing in Agrobacterium-induced tumors

ABSTRACT: rs11-03_agro transcriptomic comparison of tumoric tissues induced by different c58 agrobacterium lines What are the functions and the regulatory pathways of the bacterial quorum-sensing system in the context of Agrobacterium/Arabidopsis interactions?

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE48402 | GEO | 2013/09/01

SECONDARY ACCESSION(S): PRJNA210138

REPOSITORIES: GEO

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Transcriptomic comparison of tumoric tissues induced by different c58 agrobacterium lines; Quorum-sensing in Agrobacterium-induced tumors

Project description:rs11-03_agro transcriptomic comparison of tumoric tissues induced by different c58 agrobacterium lines What are the functions and the regulatory pathways of the bacterial quorum-sensing system in the context of Agrobacterium/Arabidopsis interactions? Arabidopsis WT Col0 plants were grown in greenhouse till stage 1.03-1.04 (growth conditions 1). They were then confined in short day conditions and at 22Â°C (growth conditions 2). At stage 5.10 plants were wounded at the base of the stem and part of them were inoculated with 3 different C58 Agrobacterium strains (WT, accR or traI). Eighteen days post inoculation, wounded but non-infected tissues (control) as well as tumoric tissues were collected and processed for RNA extraction. 21 dye-swap

2013-09-01 | E-GEOD-48402 | biostudies-arrayexpress

Agrobacterium tumefaciens C58 whole-genome tiling microarray design and validation

Project description:This research focuses on the design, manufacturing and validation of a new Agrobacterium tumefaciens C58 whole-genome tiling microarray platform for novel RNA transcript discovery. A whole-genome tiling microarray allows both annotated genes as well as previously unknown RNA transcripts to be detected and quantified at once. The Agrobacterium tumefaciens C58 genome is re-acquired with next-generation sequencing and then used to design the tilinlg microarray with the thermodynamic analysis program Picky. Validations are performed by subjecting Agrobacterium tumefaciens C58 under various growth conditions and then using the tling microarrays to verify expected gene expression patterns.

2014-09-25 | GSE61737 | GEO

Arabidopsis thaliana Col-0 root transcriptome response to Agrobacterium tumefaciens C58

Project description:As sessile organisms, plants require dynamic pathways in order to recognize pathogens and coordinate plant defenses by signalling. Agrobacterium tumefaciens C58 is able to avoid triggering plant defenses prior to entering the cell, and therefore is only detected once infection has begun making Agrobacterium a plant pathogen to numerous plant species. Understanding plant responses to Agrobacterium will be useful in improving plant defenses and potentially may also improve plant transformation efficiency. Microarrays were utilized for detailing the global gene expression pattern in A. thaliana Col-0 roots in response to A. tumefaciens C58 for the identification of differentially expressed genes.

2014-10-28 | GSE62750 | GEO

Arabidopsis thaliana Col-0 leaf transcriptome response to Agrobacterium tumefaciens C58

Project description:As sessile organisms, plants require dynamic pathways in order to recognize pathogens and coordinate plant defenses by signalling. Agrobacterium tumefaciens C58 is able to avoid triggering plant defenses prior to entering the cell, and therefore is only detected once infection has begun making Agrobacterium a plant pathogen to numerous plant species. Understanding plant responses to Agrobacterium will be useful in improving plant defenses and potentially may also improve plant transformation efficiency. Microarrays were utilized for detailing the global gene expression pattern in A. thaliana Col-0 leafs in response to A. tumefaciens C58 for the identification of differentially expressed genes.

2014-10-28 | GSE62749 | GEO

Trancriptome analysis of Agrobacterium tumefaciens C58 cells in the presence of abundant opines

Project description:Purpose: The goals of this study is to compare the reponse of Agrobacterium tumefaciens C58 in the presence and absence of the two opines nopaline and agrocinopine (more precisely agrocinopine A) to delineated the key-genes associated to opines-response in A. tumefaciens C58.

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Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii

Project description:Analysis of proteins under the influence of the quorum sensing (QS) system in the nonpathogenic Agrobacterium tumefaciens strain 6N2, and the influence of the bacterial QS system in the proteome of the yeast Meyerozyma guilliermondii strain 6N.

2021-12-20 | PXD025730 | Pride

Arabidopsis thaliana Col-0 leaf transcriptome response to Agrobacterium tumefaciens C58

Project description:As sessile organisms, plants require dynamic pathways in order to recognize pathogens and coordinate plant defenses by signalling. Agrobacterium tumefaciens C58 is able to avoid triggering plant defenses prior to entering the cell, and therefore is only detected once infection has begun making Agrobacterium a plant pathogen to numerous plant species. Understanding plant responses to Agrobacterium will be useful in improving plant defenses and potentially may also improve plant transformation efficiency. Microarrays were utilized for detailing the global gene expression pattern in A. thaliana Col-0 leafs in response to A. tumefaciens C58 for the identification of differentially expressed genes. 3-week-old A.thaliana Col-0 seedlings were selected for growth in hydroponic systems. A. tumefaciens C58 was inoculated into the hydroponic system and co-cultivation persisted for 8 hours. Leaf tissue was seperated for RNA extraction and hybridization to the ATH1 Affymetrix microarray.

2014-10-28 | E-GEOD-62749 | biostudies-arrayexpress

Arabidopsis thaliana Col-0 root transcriptome response to Agrobacterium tumefaciens C58

2014-10-28 | E-GEOD-62750 | biostudies-arrayexpress

Agrobacterium fabrum str. C58

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Comparative genomic hybridization of Agrobacterium tumefaciens strains on tiling arrays covering the genome of strain C58

Project description:Comparative genomic hybridizations obtained with an original Agrobacterium tumefaciens strain C58 genome-based micro-array were used to detect the presence or absence of genes homologous to those of strain C58 in 25 agrobacterial strains. These strains included six other members of genomovar G8, one to three strains for each of the nine other A. tumefaciens genomovars and one for A. larrymoorei, a sister species of the A. tumefaciens complex. An original probabilistic method was used to segment C58 replicon sequences into regions, that are absent or present in tested strains, allowing us to detect the presence of homologues of C58 coding sequences (CDSs) in tested strains.

2011-09-08 | E-MTAB-558 | biostudies-arrayexpress

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