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ChIP-seq in MRC5 hIPSCs overexpressing MEG3 RNA.

ABSTRACT: This experiment was designed to detect differences in PRC2 occupancy in a human induced pluripotent cell line (MRC5) naturally deficient in MEG3 RNA after lentiviral overexpression of MEG3.

ORGANISM(S): Homo sapiens

PROVIDER: GSE48515 | GEO | 2013/12/25

SECONDARY ACCESSION(S): PRJNA210418

REPOSITORIES: GEO

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ChIP-seq in MRC5 hIPSCs overexpressing MEG3 RNA.

Project description:This experiment was designed to detect differences in PRC2 occupancy in a human induced pluripotent cell line (MRC5) naturally deficient in MEG3 RNA after lentiviral overexpression of MEG3. MRC5 were transduced with lentiviruses for the overexpression of MEG3 lncRNA or GFP as a control. ChIP-seq was performed with EZH2 and JARID2 antibodies.

2013-12-25 | E-GEOD-48515 | biostudies-arrayexpress

ChIP-seq in MEG3+ and MEG3- hIPSCs

Project description:In this experiment we sought to identify region with differential PRC2, JARID2, and H3K27me3 occupancy in human induced pluripotent stem cell lines with silenced or expressed MEG3 locus to indirectly determine the effect of this ncRNA on PRC2 function. 8 hIPSC lines, 5 MEG3+ and 3 MEG3-; ChIP-seq for EZH2, JARID2, and H3K27me3. Some done in replicates.

2013-12-25 | E-GEOD-48516 | biostudies-arrayexpress

ChIP-seq in MEG3+ and MEG3- hIPSCs

2013-12-25 | GSE48516 | GEO

Expression profiling of MRC5, IFN gamma treated MRC5 and PGF cells.

Project description:Expression profiling of MRC5, IFN gamma treated MRC5 and PGF cells. Keywords: cell type comparison, IFN gamma treatment.

2008-07-01 | GSE10880 | GEO

Transcription profiling of human MRC5, IFN gamma treated MRC5 and PGF cells.

Project description:Expression profiling of MRC5, IFN gamma treated MRC5 and PGF cells. Experiment Overall Design: MRC5 cells were treated for 24 hours with IFN gamma (200 IU/ml) and their expression profile was compared to untreated MRC5 and PGF cells.

2008-10-20 | E-GEOD-10880 | biostudies-arrayexpress

Transcriptome of MRC5 fibroblasts

Project description:Purpose: to investigate the human transcriptome in MRC5 fibroblasts

2022-12-18 | GSE171519 | GEO

overexpression/knockdown MEG3 in C2C12 cell lines

Project description:MEG3 was known as a growth suppressor in tumor cells by activating p53. Besides, MEG3 could regulate transforming growth factor-β (TGF-β) signaling pathway, which is the key regulator of skeletal myogenesis and can enhance the proliferation of myogenic cells. Previous study also showed MEG3 was highly expressed in the paraxial mesoderm and probably regulated muscle development. To investigate the potential function of MEG3 in muscle development, we detected the expression levels of protein-coding genes after MEG3 over-expression or knockdown in C2C12 cell line using microarrays.

2018-09-28 | GSE73524 | GEO

Herbaspirillum sp. meg3

Project description:Herbaspirillum sp. meg3 Genome sequencing

| PRJNA397799 | ENA

Genomic alteration in MRC5-derived induced pluripotent stem (MRC5-iPS) cell line, Tic

Project description:Disease-specific induced pluripotent stem (iPS) cells have been used for a model to analyze pathogenesis of the disease. We generated iPS cells derived from a fibroblastic cell line of ataxia telangiectasia (AT-iPS cells). In analysis of AT-iPS cells, the human wild-type iPS cell line (MRC5-iPS) was generated and cultured in the same conditions as the diseased iPS cell lines. It is an ideal control cell line for the disease and patient-specific iPS cell lines. Because MRC5-iPS cells exhibited considerable chromosomal abnormalities in vitro, we performed a structural alteration analysis by using a SNP genotyping array for MRC5-iPS cell line, Tic, at passage 15, passage 30, and passage 37.

2013-07-23 | GSE49082 | GEO

Meg3 regulated genes in pancreatic beta cells

Project description:Meg3 is a long non-coding RNA. It's target genes are unknown. The mouse pancreatic beta cell line MIN6-4N was used to assess the expression of genes upon stable Meg3 overexpression Stable cell lines were isolated that have integated pcDNA3.1 or pcDNA3.1-mMeg3.The cells were processed for RNA isolation. The level of Meg3 expression was assessed by RT-PCR. RNA preps were used for microarray analysis.

2015-05-21 | E-GEOD-57716 | biostudies-arrayexpress

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