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Madin-Darby canine kidney cells expression array

ABSTRACT: cDNA expression array of epithelial phenotype untreated MDCK cells; after 30 days of TGFb treatment, which induces a mesenchymal phenotype; TGF-b-treated cells that are treated with 5-AZA for 72 h; and 30 days after TGFb withdrawal Gene expression was analyzed in a genome-wide manner, to asses those changes occurring upon TGF-b-induced epithelial to mesenchymal transition (EMT), those that are stimulated by treatment with a demethylating agent, and which are restored after TGF-b withdrawal

ORGANISM(S): Canis lupus familiaris

PROVIDER: GSE48525 | GEO | 2014/08/14

SECONDARY ACCESSION(S): PRJNA210437

REPOSITORIES: GEO

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Madin-Darby canine kidney cells expression array

Project description:cDNA expression array of epithelial phenotype untreated MDCK cells; after 30 days of TGFb treatment, which induces a mesenchymal phenotype; TGF-b-treated cells that are treated with 5-AZA for 72 h; and 30 days after TGFb withdrawal Gene expression was analyzed in a genome-wide manner, to asses those changes occurring upon TGF-b-induced epithelial to mesenchymal transition (EMT), those that are stimulated by treatment with a demethylating agent, and which are restored after TGF-b withdrawal Two replicates for untreated and TGFb treated, and one sample for 5-AZA and TGFb withdrawal

2014-08-14 | E-GEOD-48525 | biostudies-arrayexpress

Whole genome bisulfite sequencing of MDCK cells, before and after TGFB-induced epithelial-mesenchymal transition

Project description:Whole genome bisulfite sequencing of MDCK cells, before and after TGFB-induced epithelial-mesenchymal transition Sequencing of bisulfite converted DNA from MDCK cells untreated, and after a 30 days treatment with TGF beta

2014-08-14 | E-GEOD-43697 | biostudies-arrayexpress

Inter-Laboratory Variability in the Madin-Darby Canine Kidney Cell ProteomeInter-Laboratory Variability in the Madin-Darby Canine Kidney Cell Proteome

Project description:Madin-Darby Canine Kidney (MDCK) cells are widely used to study epithelial cell functionality. Their low endogenous drug transporter protein levels make them an amenable system to investigate trans-epithelial permeation and drug transporter protein activity after their transfection. MDCK cells display diverse phenotypic traits and as such laboratory-to-laboratory variability in drug permeability assessments are observed. Consequently, In Vitro-In Vivo extrapolation (IVIVE) approaches using permeability and/or transporter activity data require calibration. A comprehensive proteomic quantification of eleven filter-grown parental or mock-transfected MDCK monolayers from eight different pharmaceutical laboratories using the Total Protein Approach (TPA) is provided. TPA enabled estimations of key morphometric parameters such as monolayer cellularity and volume. Overall, metabolic liability to xenobiotics is likely to be limited for MDCK cell due to the low expression of required enzymes. SLC16A1 (MCT1) was the highest abundance SLC transporter linked to xenobiotic activity, while ABCC4 (MRP4) was the highest abundant ABC transporter. Our data supports existing findings that Claudin-2 levels may be linked to tight junction modulation, thus impacting trans-epithelial resistance. This unique database provides data on more than 8000 proteins copy number and concentrations, thus allowing an in-depth appraisal of the control monolayers used in each laboratory.

2023-07-20 | PXD039552 | Pride

Transcriptome profiling of madin-darby canine kidney cells with canine parvovirus 2a persistent infection

Project description:It has been demonstrated that parvovirus infection causing cytotoxicity and immune activation effect in many virus-induced cell lines, however, the underlying mechanisms are not fully understood. To investigate the gene expression signature of host cell after CPV-2 infection, we performed transcriptome profiling of MDCK after persistent infection of CPV-2a using microarray analysis. Uninfected MDCK cells act as negative control. The data show the genes regulated by CPV-2 infection..

2016-12-01 | GSE72397 | GEO

Madin Darby Canine Kidney (MDCK) Cells: Early & Late Passage

Project description:The paracellular passage of ions in epithelial systems is dictated by the repertoire of tight junction (TJ) proteins, the number and architecture of apicolateral TJ strands, and the interaction of TJ proteins with the cytoskeleton. To investigate the relative contribution of these factors and to explore their regulation, we examined a passage number dependent phenotypic change in Madin Darby Canine Kidney (MDCK) cells iteratively passaged at low density in which dilution potentials of the monolayer abruptly decreased but transepithelial resistance (TER) progressively increased. Here, we report that the phenotypic transition involved a decrease surface expression of peanut agglutinin (PNA), a decrease in steady state levels of claudin-1 and a slight decrease in E-cadherin expression in the context of an apparently claudin-2-negative system. Late passage cells grew in more densely packed monolayers and exhibited characteristics of a competent, functional cultured monolayer including inducible vectorial ion transport in response to norepinephrine (NE) and sphere formation in three dimensional laminin gel culture. Microarray analysis of gene expression levels in early and late passage cells revealed an increase in transcript abundance for SGK and GLUT-3, and a reduction Id-3. The steady state protein expression levels of GLUT-3, however, were found to be equivalent. These findings suggest that the expression of claudin-2 in MDCK cells could be contingent on signaling via lateral cell contacts and that claudin-1, in the context of sustained claudin-8 expression, could function as a negative regulator of overall TER.

2010-02-13 | GSE14837 | GEO

RNA-Seq of Ras induced Epithelial to Mesenchymal Transition (EMT) in Madin-Darby Canine Kidney (MDCK) cells

Project description:To gain more information about EMT induced by oncogenic Ras in MDCK cells, we performed RNA-seq of MDCK and MDCK-Ras cells and compared their genome-wide gene expression profiles.

2016-02-03 | E-MTAB-3301 | biostudies-arrayexpress

Whole genome bisulfite sequencing of MDCK cells, before and after TGFB-induced epithelial-mesenchymal transition

Project description:Whole genome bisulfite sequencing of MDCK cells, before and after TGFB-induced epithelial-mesenchymal transition

2014-08-14 | GSE43697 | GEO

Madin-Darby Canine Kidney (MDCK) cells grown in 2-dimensional and 3-dimensional type I collagen culture.

Project description:In order to gain insight into epithelial morphogenesis and the influence of culture geometry on gene expression patterns, Madin Darby Canine Kidney (MDCK) epithelial cells where grown in 2-dimensional (2D) culture or 3-dimensional (3D) culture . MDCK cells cultured in 2D were plated atop a pre-solidified type I collagen gel. Cells cultured under these conditions grew as flat monolayer sheets. In 3D culture, cells are embedded within type I collagen gel. Cells grown under these conditions form large spherical cysts with hollow central lumens. We anticipate, therefore, that these results provide insight into the mechanisms that regulate epithelial cystogenesis.

2013-06-03 | GSE28381 | GEO

microRNA-Seq of Ras induced Epithelial to Mesenchymal Transition (EMT) in Madin-Darby Canine Kidney (MDCK) cells

Project description:To gain more information on the expression of microRNAs during EMT induced by oncogenic Ras in MDCK cells, we performed deep sequencing (Illumina HiSeq2000) of MDCK and MDCK-Ras cells and compared their genome-wide microRNA expression profiles. RNA-Seq data from the same study has also been deposited at ArrayExpress under accession number E-MTAB-3301 (https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-3301/).

2015-11-17 | E-MTAB-3302 | biostudies-arrayexpress

Madin Darby Canine Kidney (MDCK) Cells: Early & Late Passage

2010-04-09 | E-GEOD-14837 | biostudies-arrayexpress

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