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Transcriptomic approach to renal toxicity for cisplatin treatment in Nrf2 WT/KO

ABSTRACT: NF-E2-related factor 2 (Nrf2), a transcription factor activated by oxidative stress, induces phase II conjugating or antioxidant enzymes. In the present study, we examined the effect of Nrf2 knockout (Nrf2 KO) on the kidney injury induced by cisplatin using cDNA microarray analyses. A transcriptomic approach enabled us to extract gene clusters which show significantly different mRNA expression patterns between wild type (WT) and Nrf2 KO mice. In the experimental set, WT vehicle (WT-Veh) and WT cisplatin (WT-Cis) groups were included in our previous GEO database (GSE35257) because the two groups were simultaneously shared with Nrf2 KO vehicle (Nrf2 KO-Veh) and Nrf2 KO cisplatin (Nrf2 KO-Cis) groups in the current report. Copyright (c) 2012 by Korea Food & Drug Administration.

ORGANISM(S): Mus musculus

PROVIDER: GSE48879 | GEO | 2014/01/01

SECONDARY ACCESSION(S): PRJNA212115

REPOSITORIES: GEO

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Transcriptomic approach to renal toxicity for cisplatin treatment in Nrf2 WT/KO

Project description:NF-E2-related factor 2 (Nrf2), a transcription factor activated by oxidative stress, induces phase II conjugating or antioxidant enzymes. In the present study, we examined the effect of Nrf2 knockout (Nrf2 KO) on the kidney injury induced by cisplatin using cDNA microarray analyses. A transcriptomic approach enabled us to extract gene clusters which show significantly different mRNA expression patterns between wild type (WT) and Nrf2 KO mice. In the experimental set, WT vehicle (WT-Veh) and WT cisplatin (WT-Cis) groups were included in our previous GEO database (GSE35257) because the two groups were simultaneously shared with Nrf2 KO vehicle (Nrf2 KO-Veh) and Nrf2 KO cisplatin (Nrf2 KO-Cis) groups in the current report. Copyright (c) 2012 by Korea Food & Drug Administration. WT and Nrf2 KO C57BL/6 mice (9 weeks old, male) were intraperitoneally injected with vehicle (saline) or a single dose of cisplatin (15 mg/kg body weight). The cDNA microarray experiments were done for the kidney of mice 3 days after treatment. Three animals were used per group. WT-Veh and WT-Cis groups were already deposited in GSE35257.

2014-01-01 | E-GEOD-48879 | biostudies-arrayexpress

Hepatic gene expression profiles after long-term high-fat diet feeding in wild type and Nrf2 knock-out mice.

Project description:To assess the role of the transcription factor NFE2 related factor 2 like 2 ( Nrf2) in the development of high-fat diet (HFD)-induced obesity and non-alcoholic HFD-induced fatty liver disease, 8 wild type (WT) and 8 Nrf2 knock-out (Nrf2-KO) C57BL6J male mice (obtained from Riken BRC, Tsukuba, Japan and originally developed by Prof. M. Yamamoto) were fed an HFD (60 kcal % fat) for 180 days. Whole genome microarray expression profiling was performed in pooled liver samples of WT and Nrf2-KO mice to identify genes that are differentially expressed between WT and Nrf2-KO mice under the stress conditions of HFD-induced obesity. Liver samples were taken from 8 wild type (WT) and 8 Nrf2 knock-out (Nrf2-KO) male mice on high-fat diet (60 kcal % fat) for 180 days. Total RNA was isolated from pooled liver samples from WT or Nrf2-KO mice to produce 4 samples each using the guanidinium thiocyanate method.

2011-11-30 | E-GEOD-33575 | biostudies-arrayexpress

Mus musculus

Project description:Transcriptomic approach to renal toxicity for cisplatin treatment in Nrf2 WT/KO

| PRJNA212115 | ENA

Transcriptome analysis of kidneys of cisplatin-induced AKI model

Project description:This study was aimed to investigate the role and underlying mechanism of TRPM2 in cisplatin nephrotoxicity. Cisplatin-induced acute kidney injury (AKI) model was established in WT and TRPM2-KO mice. The transcriptome profiling of the kidneys of WT and TRPM2-KO mice treated with cisplatin was compared to find differentially expressed gene which may be related to TRPM2 on cisplatin nephrotoxicity.

2023-03-30 | GSE203584 | GEO

Hepatic gene expression profiles after long-term high-fat diet feeding in wild type and Nrf2 knock-out mice.

2011-11-30 | GSE33575 | GEO

DIA proteomic analysis of unstimulated murine bone marrow derived macrophages from WT, Nrf2 KO and Keap1 KD cells

Project description:Unstimulated murine bone marrow derived macrophages cultured in L92 media from WT (4 biological replicates), Nrf2 KO (3 biological replicates) and Keap1 KD BMDM (3 biological replicates) were processed and analysed utilising DIA (label free) proteomic analysis. The Nrf2 KO mouse (DOI: 10.1006/bbrc.1997.6943 ) and Keap1 KD mouse ( DOI: 10.1128/MCB.01591-09) were previously published as noted.

2022-04-01 | PXD030455 | Pride

Comparison of expression profiles of WT and Nrf2AY/AY mutant macrophages under electrophilic stress agent [RNA-Seq]

Project description:Nrf2(A502Y) mutant failed to recognize cis-element required for induction of Nrf2 target genes.To examine whether Nrf2(A502Y) supports expression of a distinct gene set from that supported by Nrf2, RNA-Seq analysis was performed. We compared gene expression profiles in peritoneal macrophages from Nrf2+/+ and Nrf2AY/AY mice between basal and DEM (which is an electorophilic stress agent promoting Nrf2 induction)-treated conditions. mRNA profiles of wild type (WT) and Nrf2AY/AY (AY) mutant macrophages under basal and DEM-treated condition were generated by deep sequencing, in triplicate.

2016-01-04 | E-GEOD-75176 | biostudies-arrayexpress

Gene expression change in placental labyrinth zone from Pregnancy-Associated Hypertension (PAH) mice with different Nrf2 expression.

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Therapeutic efficacy of INX-315, a selective CDK2 inhibitor, in solid tumors [HER2mouse resistant RNAseq]

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Endothelial ER-alpha Promotes Glucose Tolerance by Enhancing Endothelial Insulin Transport to Skeletal Muscle

Project description:Human aortic endothelial cells were treated with vehicle or estradiol. Immunoprecipitation was then performed with anti-estrogen receptor alpha antibody or mock IgG, and liquid chromatography/tandem mass spectrometry was done. 996859: Mock-IgG-Veh#1; 996860: Mock-IgG-E2#1; 996861: ERa-Veh#1; 996862: ERa-E2#1; 996863: Mock-IgG-Veh#2; 996864: Mock-IgG-E2#2; 996865: ERa-Veh#2; 996866: ERa-E2#2; 996867: Mock-IgG-Veh#3; 996868: Mock-IgG-E2#3; 996869: ERa-Veh#3; 996870: ERa-E2#3

2023-01-19 | MSV000091095 | MassIVE

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