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Genome-wide analysis of transcription factor PfNF-YB in Plasmodium falciparum.

ABSTRACT: PfNF-YB is a transcription factor of Plasmodium falciparum and is known as a CCAAT-box-binding subunit B. PfNF-YB belongs to NF-Y complex family that is conserved from yeast to human. PfNF-YB is highly expressed in schizont stage and co-localized at the nucleus in mature form during intraerythrocytic stage. We found that melatonin and cAMP second messenger can modulate the PfNF-YB expression in P. falciparum. To better understand the function of PfNF-YB in P. falciparum we proposed to perform chromatin immunoprecipitation (ChIP) of PfNF-YB together with chromatin profiling by ChIP-on-chip analysis. The results showed that PfNF-YB binds to CCAAT of several genes in schizont stage.

ORGANISM(S): Plasmodium falciparum

PROVIDER: GSE48894 | GEO | 2013/07/16

SECONDARY ACCESSION(S): PRJNA212139

REPOSITORIES: GEO

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Genome-wide analysis of transcription factor PfNF-YB in Plasmodium falciparum.

Project description:PfNF-YB transcription factor is highly expressed and co-localized at the nucleuos in mature form during intra-erytrocytic stages. melatonin and cAMP second messenger can modulate the PfNF-YB expression in P. falciparum erythrocyte stages. Chromatin immunoprecipitation (ChIP) of PfNF-YB together with chromatin profiling by ChIP-on-chip analysis demonstrated that PfNF-YB binding to CCAAT of several genes in schizont stage. comparison of PfNF-YB vs control beads and IgG

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