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Expresion analyses of red2 deletion cells

ABSTRACT: Investigation of whole genome gene expression level changes in red2∆, compared to the parental wild-type strain. The red2 deletion cells analyzed in this study will be described in Sugiyama and Sugioka-Sugiyama.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE49006 | GEO | 2013/12/01

SECONDARY ACCESSION(S): PRJNA212563

REPOSITORIES: GEO

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Expresion analyses of red2 deletion cells

Project description:Investigation of whole genome gene expression level changes in red2?, compared to the parental wild-type strain. The red2 deletion cells analyzed in this study will be described in Sugiyama and Sugioka-Sugiyama. An mRNA profiling study using total RNA recovered from vegetatively growing wild-type culture of fission yeast and red2 deletion cultures of fission yeast. Each chip measures the expression level of 4.997 genes from S.pombe with fourteen 60-mer probe pairs (PM/MM) per gene, with seven-fold technical redundancy.

2013-12-01 | E-GEOD-49006 | biostudies-arrayexpress

Expression analyses of red5-2 mutant cells

2013-05-15 | GSE44714 | GEO

Expression analyses of red5-2 mutant cells

Project description:Investigation of whole genome gene expression level changes in red5-2, compared to the wild-type strain. The mutants analyzed in this study will be described in Sugiyama and Sugiyama. A mRNA profiling study using total RNA recovered from wild-type culture of fission yeast and red5-2 mutant cultures of fission yeast. Each chip measures the expression level of 4.997 genes from S.pombe with fourteen 60-mer probe pairs (PM/MM) per gene, with seven-fold technical redundancy.

2013-05-15 | E-GEOD-44714 | biostudies-arrayexpress

Expresion analyses of red1∆

Project description:Investigation of whole genome gene expression level changes in red1∆, compared to the wild-type strain.

2011-03-01 | GSE23083 | GEO

Expresion analyses of red1∆

Project description:Investigation of whole genome gene expression level changes in red1∆, compared to the wild-type strain. A chip study using total RNA recovered from two separate wild-type cultures of fission yeast and two separate red1∆ cultures of fission yeast. Each chip measures the expression level of 1997 genes from S.pombe with fourteen 60-mer probe pairs (PM/MM) per gene, with seven-fold technical redundancy.

2011-03-01 | E-GEOD-23083 | biostudies-arrayexpress

Expression analysis of Trichoderma reesei QM9414 and delta-phlp1, delta-gnb1 and delta-gng1 in light and darkness.

Project description:Investigation of whole genome gene expression level changes in response to different light conditions of the T. reesei QM9414 parental strain and the deletion strains delta-phlp1, delta-gnb1 and delta gng1, cultivated on 1 % microcrystalline cellulose. The mutants analyzed in this study are further described in Tisch et al. 2011: Carbohydrate degradation is significantly regulated by light and the phosducin like protein PhLP1 in Trichoderma reesei (Hypocrea jecorina).

2011-03-02 | GSE27581 | GEO

Dual RNA-seq on Klebsiella pneumoniae infection to human epithelial cells

Project description:Klebsiella pneumoniae poses significant threat to global health. Detailed investigation to the nature of its interaction to human epithelial cells is still lacking. OmpR is an important regulator to the expression of major outer membrane protein genes, ompF and ompC. Here, we exploited the recently described dual RNA-seq to simultaneously measure genome-wide expression of host and pathogen two hours into infection. By comparing OmpR deleted strain to its wildtype parental strain, we, simultaneously, reconstructed OmpR regulon and deletion effect to host response.

2021-12-14 | GSE123964 | GEO

Expression analysis of Trichoderma reesei QM9414 and delta-phlp1, delta-gnb1 and delta-gng1 in light and darkness.

2011-03-02 | E-GEOD-27581 | biostudies-arrayexpress

Haloferax volcanii Proteome Response to Deletion of a Rhomboid Protease Gene

Project description:Rhomboids are conserved intramembrane serine proteases involved in cell signaling processes. Their role in prokaryotes is scarcely known and remains to be investigated in Archaea. We previously constructed a rhomboid homologue deletion mutant (ΔrhoII) in Haloferax volcanii, which showed reduced motility, increased novobiocin sensitivity, and an Nglycosylation defect. To address the impact of rhoII deletion on H. volcanii physiology, the proteomes of mutant and parental strains were compared by shotgun proteomics. A total of 1847 proteins were identified (45.8% of H. volcanii predicted proteome), from which 103 differed in amount. Additionally, the mutant strain evidenced 99 proteins with altered electrophoretic migration, which suggested differential post-translational processing/modification. Integral membrane proteins that evidenced variations in concentration, electrophoretic migration, or semitryptic cleavage in the mutant were considered as potential RhoII targets. These included a PrsW protease homologue (which was less stable in the mutant strain), a predicted halocyanin, and six integral membrane proteins potentially related to the mutant glycosylation (S-layer glycoprotein, Agl15) and cell adhesion/motility (flagellin1, HVO_1153, PilA1, and PibD) defects. This study investigated for the first time the impact of a rhomboid protease on the whole proteome of an organism.

2020-05-14 | PXD011218 | Pride

Expression analysis of Thermococcus onnurineus NA1 KCTC10859 and an Frh-deficient mutant, Frh overexpression mutant and TON_0282 deletion mutant in a T. onnurineus NA1 strain.

Project description:Investigation of whole genome gene expression level changes in an Frh-deficient mutant, Frh overexpression mutant and TON_0282 deletion mutant, compared to the wild-type strain. In this study, as a first step to assess the role of the frhAGB-encoding hydrogenases and TON_0282 gene, we constructed three mutants by deleting each gene in a T. onnurineus NA1.

2018-02-19 | GSE88718 | GEO

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