Project description:Dichaete is a developmentally important transcription factor, known to be involved in basic biological processes including segmentation and nervous system development among others. The aim of this experiment was to gain further insight into the role of Dichaete during early embryogenesis, by looking at the disruption of gene expression in Dichaete mutants. Stage 10-11 embryos (5 and 7.5 hours after egg laying) from a cross between Dr72/TM3, twi-GAL4 UAS-Gfp Dr513/TM3, twi-GAL4 UAS-Gfp, were hand picked under a fluorescence dissecting microscope. GFP negative homozygous Dichaete mutant embryos and their heterozygous single GFP positive siblings were collected and approximately 150 embryos per sample were stored frozen in Trizol

2013-07-22 | E-GEOD-49069 | biostudies-arrayexpress

The role of Dichaete in transcriptional regulation the of Drosophila midline [simgal4]

2013-07-22 | GSE49094 | GEO

The role of Dichaete in transcriptional regulation the of Drosophila midline [prosGal4]

2013-07-22 | GSE49071 | GEO

Functional role of miRNAs in the renal stroma during embryonic kidney development

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-11-04 | E-GEOD-64442 | biostudies-arrayexpress

The role of Dichaete in transcriptional regulation the of Drosophila midline [simgal4]

Project description:Dichaete is a developmentally important transcription factor, known to be involved in basic biological processes including segmentation and nervous system development among others. The aim of this experiment was to gain further insight into the role of Dichaete during early embryogenesis, specifically looking at targets in the midline using dominant negative constructs expressed via the UAS/Gal4 system, using simGal4 to drive expression in the midline. 4 independent biological replicates. 3.5-4.5h old embryos were collected, and the RNA was extracted using Trizol. The UAS-dominant negative construct expressing embryos were compared to UAS-GFP expressing controls. Construct expression was driven using simgal4.

2013-07-22 | E-GEOD-49094 | biostudies-arrayexpress

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