Transcriptomics,Genomics

Dataset Information

32

Gene expressional comparison of in vitro adipocyte models vs. in vivo eWAT


ABSTRACT: Comparison of gene expression level of 3T3-L1, PMEF and ES cell derived adipocytes to eWAT samples. We used microarray to investigate the gene expressional differences between different cellular models of adipocyte differentiation vs. in vivo eWAT tissues Overall design: 3T3-L1 cells, Primary Mouse Embryonic Fibroblasts (PMEFs) and mouse embryonic stem cells (mESCs) were differentiated to adipocytes as it is described in details in the manuscript. eWAT tissues has been isolated from healthy wild-type mice. Each in biological triplicates.

INSTRUMENT(S): [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]

SUBMITTER: Zoltan Simandi  

PROVIDER: GSE49176 | GEO | 2014-12-01

SECONDARY ACCESSION(S): PRJNA213230

REPOSITORIES: GEO

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Publications

Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid.

Cuaranta-Monroy Ixchelt I   Simandi Zoltan Z   Kolostyak Zsuzsanna Z   Doan-Xuan Quang-Minh QM   Poliska Szilard S   Horvath Attila A   Nagy Gergely G   Bacso Zsolt Z   Nagy Laszlo L  

Stem cell research 20140505 1


Adipocyte differentiation and function have become the major research targets due to the increasing interest in obesity and related metabolic conditions. Although, late stages of adipogenesis have been extensively studied, the early phases remain poorly understood. Here we present that supplementing ascorbic acid (AsA) to the adipogenic differentiation cocktail enables the robust and efficient differentiation of mouse embryonic stem cells (mESCs) to mature adipocytes. Such ESC-derived adipocytes  ...[more]

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