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Real-time quantitative PCR analysis of microRNA expression in neonatal and adult cardiovascular progenitors

ABSTRACT: Human cardiovascular stem cells were isolated from adult (57-75 year old) and neonatal (<1 month old) atrial tissue. Cardiovascular stem cells were then cloned by single cell dilution and compared using sabiosciences cell development & differentiation miRNA PCR array.

ORGANISM(S): Homo sapiens

PROVIDER: GSE49235 | GEO | 2014/08/31

SECONDARY ACCESSION(S): PRJNA213327

REPOSITORIES: GEO

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Real-time quantitative PCR analysis of microRNA expression in neonatal and adult cardiovascular progenitors

Project description:Human cardiovascular stem cells were isolated from adult (57-75 year old) and neonatal (<1 month old) atrial tissue. Cardiovascular stem cells were then cloned by single cell dilution and compared using sabiosciences cell development & differentiation miRNA PCR array. microRNA expression profiling by RT-PCR, 3 cardiac progenitor cell clones isolated from adults were run separately and results were pooled and compared to 8 neonatal cardiovascular progenitor cell clones that were run separately and pooled.

2014-08-31 | E-GEOD-49235 | biostudies-arrayexpress

Real-time quantitative PCR analysis of microRNA expression in neonatal cardiovascular progenitors before and after 7 Days of simulated microgravity.

Project description:Human cardiovascular progenitors were isolated from neonatal (<1 month old) atrial tissue and cloned by single cell dilution. MicroRNA expression in neonatal cardiovascular progenitor cell (CPC) clones was compared before and after exposure to simulated microgravity using sabiosciences cell development & differentiation miRNA PCR array.

2016-02-01 | GSE65795 | GEO

Real-time quantitative PCR analysis of the expression of cytoskeleton regulator genes in 4 week old male MeCP2 A140V mutant mouse cerebellum

Project description:The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Cytoskeleton Regulators Array (Catalog# PAMM-088A) from SABiosciences. Mice used in this study were 4 week-old males.

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Real-time quantitative PCR analysis of the expression of cytoskeleton regulator genes in 2 week old male MeCP2 A140V mutant mouse cerebellum

Project description:The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates. The PCR array used for these experiments was the Mouse Cytoskeleton Regulators Array (Catalog# PAMM-088A) from SABiosciences. Mice used in this study were 2 week old males.

2015-02-01 | GSE35690 | GEO

Real-time quantitative PCR analysis of the expression of cytoskeleton regulator genes in 4 week old male MeCP2 A140V mutant mouse brain cortex

Project description:The goal of this study was to identify brain cortex gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Cytoskeleton Regulators Array (Catalog# PAMM-088A) from SABiosciences. Mice used in this study were 4 week old males.

2015-02-01 | GSE35770 | GEO

Real-time quantitative PCR analysis of the expression of cytoskeleton regulator genes in 2 week old male MeCP2 A140V mutant mouse brain cortex

2015-02-01 | GSE35769 | GEO

Real-time quantitative PCR analysis of the expression of chromatin modification enzyme genes in 4 week-old male MeCP2 A140V mutant mouse cerebellum

Project description:The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Epigenetic Chromatin Modification Enzymes Array (Catalog# PAMM-085A) from SABiosciences. Mice used in this study were 4 week old males.

2015-02-01 | GSE35689 | GEO

Real-time quantitative PCR analysis of the expression of chromatin modification enzyme genes in 2 week-old male MeCP2 A140V mutant mouse cerebellum

Project description:The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates. The PCR array used for these experiments was the Mouse Epigenetic Chromatin Modification Enzymes Array (Catalog# PAMM-085A) from SABiosciences. Mice used in this study were 2 week old males.

2015-02-01 | GSE35688 | GEO

Real-time quantitative PCR analysis of the expression of neurotransmitter receptor and regulator genes in 4 week-old male MeCP2 A140V mutant mouse cerebellum

Project description:The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Neurotransmitter Receptors and Regulators Array (Catalog# PAMM-060A) from SABiosciences. Mice used in this study were 4 week old males.

2015-02-01 | GSE35693 | GEO

Real-time quantitative PCR analysis of the expression of neurotransmitter receptor and regulator genes in 2 week-old male MeCP2 A140V mutant mouse cerebellum

Project description:The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Neurotransmitter Receptors and Regulators Array (Catalog# PAMM-060A) from SABiosciences. Mice used in this study were 2 week old males.

2015-02-01 | GSE35692 | GEO

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