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Gene expression on in vitro activated DCs

ABSTRACT: We have analyzed the effects of IL-27 signaling in dendritic cells (DCs) in the activation and polarization of effector and regulatory T cells, and the development of experimental autoimmune encephalomyelitis, an experimental model of multiple sclerosis. In this dataset, we include the results of treating DCs with IL-27.

ORGANISM(S): Mus musculus

PROVIDER: GSE49328 | GEO | 2013/07/30

SECONDARY ACCESSION(S): PRJNA213636

REPOSITORIES: GEO

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Gene expression on in vitro activated DCs

Project description:We have analyzed the effects of IL-27 signaling in dendritic cells (DCs) in the activation and polarization of effector and regulatory T cells, and the development of experimental autoimmune encephalomyelitis, an experimental model of multiple sclerosis. In this dataset, we include the results of treating DCs with IL-27. Primary splenic C57BL/6 DCs were isolated, cultured in vitro +/- IL-27 and RNA was prepared.

2013-07-30 | E-GEOD-49328 | biostudies-arrayexpress

Cholera toxin mediates cAMP-dependent CTLA-2 secretion by dendritic cells to induce regulatory T cells in the EAE model

Project description:Therapy of autoimmune diseases by tolerogenic dendritic cells (DCs) represents an interesting option for clinical application. Mostly, immature or semi-mature stages of DCs have been shown to convert naïve T cells into FoxP3+ induced regulatory T cells (iTreg). Here we found that murine bone marrow-derived DCs (BM-DCs) matured with cholera toxin (CT) produced cytotoxic T lymphocyte antigen 2 (CTLA-2) via cAMP signaling. CTLA-2 is a cathepsin L inhibitor, however, it exerted cathepsin L-independent tolerogenic functions as shown with cathepsin L knock-out BM-DCs. High or low dose CT (CThi, CTlo) or cAMP treatment of BM-DCs, but not other stimuli such as LPS or TNF, induced mRNA of both isoforms of CTLA-2α and CTLA-2β. CThi-, CTlo- or cAMP-DCs display a mature phenotype (MHC IIhi, costimulationhi). Importantly, only CThi DCs secreted IL-1β, IL-6 and IL-23. Consequently, CThi DCs instructed Th17 cell polarization while, CTlo- or cAMP-DCs induced TGF-β-dependent FoxP3+ iTreg conversion but little or no Th17 cells. iTreg conversion could be reduced by blocking of CTLA-2 with siRNAs and in accordance, addition of recombinant CTLA-2α increased the conversion towards iTreg in vitro. Injection of CTlo- or cAMP-DCs exerted MOG peptide-specific protective effects in experimental autoimmune encephalomyelitis (EAE). Together, we identified CTlo- or cAMP-induced CTLA-2 production by BM-DCs as a tool to enhance their iTreg induction in vitro and in vivo. These findings point to CTLA-2 as a novel mediator of immune evasion by cholera bacteria and its potential use to treat autoimmune diseases.

2017-03-15 | GSE79274 | GEO

Comparative genomic hybridization of rat SJL sub-strains of an experimental autoimmune encephalomyelitis (EAE) rodent model of Multiple Sclerosis (MS)

Project description:Copy number variation in two SJL sub-strains of an experimental autoimmune encephalomyelitis (EAE) rodent model of Multiple Sclerosis (MS)

2010-02-26 | E-MEXP-2534 | biostudies-arrayexpress

Peli1 knock-out and wild type mouse brain proteomes during the disease course of experimental autoimmune encephalomyelitis

Project description:The ubiquitin ligase Peli1 has previously been suggested as a potential treatment target in multiple sclerosis since the knock-out induced less activated microglia and less inflammation in the CNS of experimental autoimmune encephalomyelitis in mice. In the present study the brain proteomes of Peli1 knock-out mice and wild-type mice were analyzed and compared before disease induction and after 10 and 20 days of experimental autoimmune encephalomyelitis using quantitative proteomics. The brain samples were analyzed using TMT labeling of small pools of samples and verified using label-free of individual mice.

2016-09-27 | PXD003710 | Pride

Naive, EAE placebo-treated and EAE 1,25(OH)2D3-treated groups

Project description:B10.PL mice with severe (stage 2.5-3) experimental autoimmune encephalomyelitis were treated with placebo or 200 ng 1,25(OH)2D3. Six hours later, the spinal cords were harvested and mRNA was extracted for microarray analysis. Naive mice serve as controls. Individual samples were hybridized to individual microarrays. Keywords = Experimental autoimmune encephalomyelitis Keywords = multiple sclerosis Keywords = 1 Keywords = 25(OH)2D3 Keywords: repeat sample

2003-12-01 | GSE842 | GEO

Data in support of Identification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with Etomoxir or Interferon-beta

Project description:Multiple Sclerosis is a neurogenerative disease, where inflammation plays an essential role and the loss of tolerance for self produces proteins is a key feature. To investigate the autoimmune response, we use serum from experimental autoimmune encephalomyelitis (EAE) induced rats and investigate autoantibody chance in connection with induced disease and treatment with Etomoxir or Interferon-beta. Several significant differences in suggested antigens were identified comparing healthy, diseased and treated groups, suggesting a role of these antigens in the progression of Multiple Sclerosis.

2018-05-14 | PXD008835 | Pride

Effect of Clostridia strains on EAE clinical outcome as a therapeutic treatment

Project description:Researchers have induced an experimental model of multiple sclerosis [experimental autoimmune encephalomyelitis (EAE)] in mice to investigate the therapeutic effect of the oral treatment with selected Clostridia strains on the clinical outcome of EAE and its mechanisms of action

2021-09-15 | GSE155438 | GEO

Effect of Compound Edaravone on Experimental Autoimmune Encephalitis Mice Lumbar Spinal Cord

Project description:Researchers have induced an experimental model of multiple sclerosis [experimental autoimmune encephalomyelitis (EAE)] in C57BL/6 mice to test the neuro-therapeutic effect of Compound Edaravone (CEDA, Jiangsu Simcere Pharmaceutical Co., Ltd., Nanjing, China) during the disease peak and investigate its possible mechanisms

2022-12-31 | GSE186318 | GEO

lncRNA and mRNA expression signatures for experimental autoimmune encephalomyelitis model mice and normal mice

Project description:To explore the potential roles of lncRNAs in the progress of multiple sclerosis, spleens of experimental autoimmune encephalomyelitis (EAE) model mice and normal mice were used as the samples to obtain the expression profiles of lncRNAs and mRNAs with the mouse LncRNA Array v3.0.

2020-06-04 | GSE151701 | GEO

Mouse brain proteome map of Experimental autoimmune encephalomyelitis (EAE) mouse model

Project description:Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS); its cause is unknown. To understand the pathogenesis of MS, researchers often use the experimental autoimmune encephalomyelitis (EAE) mouse model. Here, our aim was to build a proteome map of the biological changes that occur during MS at the major onset sites—the brain and the spinal cord. We performed quantitative proteome profiling in five specific brain regions and the spinal cord of EAE and healthy mice with high-resolution mass spectrometry based on tandem mass tags.

2019-02-25 | PXD011428 | Pride

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