Blastocyst Formation
Ontology highlight
ABSTRACT:
ORGANISM(S): Bos taurus
PROVIDER: GSE49391 | GEO | 2014/01/30
SECONDARY ACCESSION(S): PRJNA213878
REPOSITORIES: GEO
Similar Datasets
Project description:In this study we aimed to examine the effect of alternative in vivo and in vitro culture conditions during the time of blastocyst formation on the transcriptome profile of bovine blastocysts. Two different blastocyst groups were produced. The first group (Vitro_morula) was matured, fertilized and cultured in vitro until morula stage then transferred to synchronized recipients and blastocysts were collected at day 7 by uterine flushing. The second group (Vivo_morula) was matured, fertilized and cultured in vivo until morula stage then flushed out and cultured in vitro until day 7. Complete in vitro (IVP) and in vivo blastocysts were produced and used as controls.
2014-01-30 | E-GEOD-49391 | biostudies-arrayexpress
Project description:There is an inevitable need for combining in vitro and in vivo culture systems during specific developmental time points to facilitate a comprehensive understanding of early embryo development which would yield insights into the molecular pathways controlling early development and to improve our knowledge in regulation of embryonic development. In current study, we aimed to understand the influences of alternative culture conditions (in vivo or in vitro) during EGA event on embryonic developmental rate, gene expression pattern and subsequent influences on pathways and biological functions controlling bovine embryo development. Six different blastocyst groups were produced under alternative in vivo and in vitro culture conditions. The first two groups (Vitro_4-cell and Vitro_16-cell) were matured, fertilized and cultured in vitro until 4- and 16-cell stage, respectively then transferred to synchronized recipients and continued culture in vivo until day 7 blastocyst stage. The second two groups (Vivo_4-cell and Vivo_16-cell) were matured, fertilized and cultured in vivo until 4- and 16-cell stage, respectively then flushed out and cultured in vitro until day 7 blastocyst stage. Complete in vitro (IVP) and in vivo (control group) blastocysts were also produced. Samples from the three pools (biological replicates) of each blastocyst group and in vivo control blastocyst were hybridized on EmbryoGENE’s bovine microarray using a dye-swap design (technical replicates) for a total of six arrays.
2012-02-29 | E-GEOD-33314 | biostudies-arrayexpress
Project description:Understanding blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. We developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via the assembly of trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, and represent an accessible in vitro model for studying bovine embryogenesis.
2023-05-16 | GSE221248 | GEO
Project description:There is an inevitable need for combining in vitro and in vivo culture systems during specific developmental time points to facilitate a comprehensive understanding of early embryo development which would yield insights into the molecular pathways controlling early development and to improve our knowledge in regulation of embryonic development. In current study, we aimed to understand the influences of in vitro culture conditions on bovine blastocyst originated in vivo at 2- or 8-cell stagre. The effect of this altrnative cuture conditions on developmental rate, gene expression pattern and subsequent influences on pathways and biological functions controlling bovine embryo development has been analyzed.
2018-10-31 | GSE111990 | GEO
Project description:Functional profile of bovine blastocyst-derived trophoblast cells
2018-11-13 | MSV000083135 | MassIVE
Project description:In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act intra-cellularly by inhibiting GSH synthesis (0.4 mM buthionine sulfoximine [BSO]) was added from days 3 to 7, and transcriptomic analysis was then performed in resulting blastocysts. Precisely, after in vitro maturation and fertilization, bovine zygotes were culture in vitro in normal condition, then at day 3, embryos were allocated into culture in control or supplemented with BSO (0.4 mM) until day 7. At this time, blastocysts were harvested and analyzed. Our hypothesis was that BSO treatment will affect blastocyst survival and gene expression associated with low embryo quality
2012-11-14 | GSE42280 | GEO
Project description:We characterzised global changes in gene expresseion between 8 cell embryos and blastocysts to identify potential genes required for blastocyst formation. Expression data from mouse 8-cell embryos and blastocysts. Eight-cell embryos and blastocysts were obatained from B6D2F1 female X B6D2F1 male. We analyzed a total of four samples at both stages.
2012-12-01 | E-GEOD-41925 | biostudies-arrayexpress
Project description:Mammalian embryogenesis begins with a totipotent zygote. Early embryogenesis can be recapitulated by aggregated extended pluripotent stem cells (EPSCs) in a 3D culture system. However, the efficiency of generating blastoids is low and whether other reported totipotent stem cells retained a similar capacity remains unknown. Here we show that spliceosomal repressed totipotent blastomere-like cells (TBLCs) form blastocyst-like structures when aggregated in 3D microwells with around 80% efficiency. TBLC-blastoids resemble blastocysts in morphology and cell-lineage allocation, show similar transcriptional profile with natural blastocysts and contain more TE cells and fewer undefined intermediate cells compared to blastoids from EPSCs. Moreover, TBLC-blastoids can develop beyond the implantation stage in vitro and induce decidualization after transferred into uterus. In summary, we supply an alternative cell type to generate ameliorated blastoids highly efficiently for studying early mouse embryogenesis.
2022-11-08 | GSE197779 | GEO
Project description:Bovine IVP blastocysts (day 8) were produced with and without thyroid hormones and gene expression profiles were compared. The IVC media (SOF) was supplemented with 50ng/ml of each T3 and T4. Four independent IVF run, each gave 1 control and 1 treated sample. Each sample is a pool of 5 blastocyst. Total Rna was extracted from each sample, amplified, labeled (also with dye swap) and hybridized to the EmbryoGene custom Agilent array (GPL13226). FlexArray software was used for data analysis.
2014-09-01 | E-GEOD-58901 | biostudies-arrayexpress
Project description:In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act extra-cellularly by promoting ROS production (0.01 mM 2,2'-azobis (2-amidinopropane) dihydrochloride [AAPH]) was added from days 3 to 7, and transcriptomic analysis was then performed in resulting blastocysts. Precisely, after in vitro maturation and fertilization, bovine zygotes were culture in vitro in normal condition, then at day 3, embryos were allocated into culture in control or supplemented with AAPH (0.01 mM) until day 7. At this time, blastocysts were harvested and analyzed. Our hypothesis was that AAPH treatment will affect blastocyst survival and gene expression associated with low embryo quality
2012-11-14 | GSE42279 | GEO