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Expression data from pancreatic cancer cell line MiaPaca2 [control]

ABSTRACT: tumor-stroma crosstalk drives pancreatic carcinogenesis we used time-resolved genome-wide transcriptional profiling to analyse changes caused by co-exposure of pancreatic tumor and stellate cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE49584 | GEO | 2013/08/09

SECONDARY ACCESSION(S): PRJNA214660

REPOSITORIES: GEO

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			Action	DRS
		Other
	GSE49584_RAW.tar	Raw
	GSE49584_normalize_filtered_TC_control_Rogon.txt.gz	Txt
	filelist.txt	Txt

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Expression data from pancreatic cancer cell line MiaPaca2

2013-08-09 | GSE49586 | GEO

Expression data from pancreatic cancer cell line MiaPaca2 [control]

Project description:tumor-stroma crosstalk drives pancreatic carcinogenesis we used time-resolved genome-wide transcriptional profiling to analyse changes caused by co-exposure of pancreatic tumor and stellate cells pancreatic tumor cell line MiaPaca2 was treated with a supernatant of pancreatic stellate cells and harvested hourly at 1-7, and 24 hours post exposure for RNA extraction and hybridization on Affymetrix microarrays.

2013-08-09 | E-GEOD-49584 | biostudies-arrayexpress

Expression data from pancreatic cancer cell line MiaPaca2

Project description:tumor-stroma crosstalk drives pancreatic carcinogenesis we used time-resolved genome-wide transcriptional profiling to analyse changes caused by co-exposure of pancreatic tumor and stellate cells pancreatic tumor cell line MiaPaca2 was treated with a supernatant of pancreatic stelalte cells, primed with cumulative TC-supernatant (of 8 tumor cell lines, TC) and harvested hourly at 1-7, and 24 hours post exposure for RNA extraction and hybridization on Affymetrix microarrays.

2013-08-09 | E-GEOD-49586 | biostudies-arrayexpress

Expression data from pancreatic stellate cells

2013-08-09 | GSE49583 | GEO

Expression data from pancreatic stellate cells

Project description:tumor-stroma crosstalk drives pancreatic carcinogenesis we used time-resolved genome-wide transcriptional profiling to analyse changes caused by co-exposure of pancreatic tumor and stellate cells Primary pancreatic Stellate cells (PSC) were treated with a cumulative supernatant of pancreatic tumor cell lines (n=8) and harvested at 1-7, and 24 hours post exposure for RNA extraction and hybridization on Affymetrix microarrays. The 8 tumor cell lines are pancreatic ductal adenocarcinoma lines: AsPC1, BxPC3, Capan1, Colo357, MiaPaca2, Panc1, Su8686, and T3M4

2013-08-09 | E-GEOD-49583 | biostudies-arrayexpress

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Project description:Pancreatic ductal adenocarcinoma (PDAC) is characterized by extensive tumor-stroma interactions that influence disease progression and therapy response. To investigate transcriptional changes associated with chemotherapy-induced tumor-stroma signaling, RNA sequencing was performed in a supernatant transfer system. Murine pancreatic cancer cells (KPC4) were treated with gemcitabine, and supernatants from gemcitabine-treated or vehicle-treated tumor cells were transferred to murine pancreatic stellate cells (PSC4). In a second experiment, supernatants from PSC4 cells previously exposed to tumor cell supernatants were transferred back to pancreatic cancer cells. RNA sequencing was performed to characterize gene expression changes associated with chemotherapy-induced tumor-stroma communication.

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