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Sugarcane transcriptome - Response to herbivory by Diatraea saccharalis

ABSTRACT: Sugarcane stalk borer larvae were grown on artificial diet and maintained at 25°C and 60±10% relative humidity with a 14 h/10 h light/dark cycle. Second instar larvae were maintained under fasting conditions for 18 h and transferred to two-month old plants (genotype SP80-3280, CTC, Brazil). Leaves were collected after 30 min and 24 h of exposure to herbivory for the control and experimental groups. Two plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of stress response

ORGANISM(S): Saccharum officinarum Saccharum hybrid cultivar SP80-3280

PROVIDER: GSE4967 | GEO | 2007/05/20

SECONDARY ACCESSION(S): PRJNA95467

REPOSITORIES: GEO

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Sugarcane transcriptome - MeJa treatment

Project description:Sugarcane one-eyed seed sets (genotype SP80-3280, CTC, Brazil) were planted in 200 ml plastic cups containing a commercial planting mix (Plantmax, Eucatex) and cultivated for 20 days under greenhouse conditions. The plantlets were subsequently transferred to a growth chamber at 26°C on a 16 h/8 h light/dark cycle with a photon flux density of 70 µE.m-2.s-1 to acclimate. Plantlets were then sprayed with a 100 µmol.L-1 MeJA solution (Bedoukian Research Inc., Danbury, CT), whereas control plantlets were treated with distilled water. Leaves were collected 0, 1, 6and 12 h after exposure to MeJA and immediately frozen in liquid nitrogen. Six plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of hormone treatment

2007-05-20 | GSE4969 | GEO

Sugarcane transcriptome: Lxx inoculated (LxxI) vs. mocked inoculated (MI) plants

Project description:Transcriptional profile of sugarcane plants variety SP80-3280 inoculated with the pathogen Leifsonia xyli subsp. xyli (Lxx) compared with mocked inoculated ones 30 and 60 days after inoculation (DAI). Goal was to determine the effects of the increase in Lxx title on global sugarcane gene expression.

2017-11-13 | GSE87826 | GEO

Vast differences in organ-specific rhythms of transcription in field-grown sugarcane

Project description:Sugarcane (Saccharum hybrid, SP80-3280) was grown in the field in Araras (Brazil) for 9 months. Leaves +1 (F1), internodes 1&2 (I1), and internodes 5 (I5) were harvested every 2 h for 26 h, starting 2h before dawn.

2019-04-10 | GSE129543 | GEO

Sugarcane transcriptome - Phosphate starvation

Project description:Rooted sugarcane plantlets, originated from in vitro meristem culture (genotype SP80-3280, CTC, Brazil), were greenhouse acclimatized by initial cultivation on 1/20th strength Hoagland and Arnon (1950) nutrient solution. Nutrient solutions were aired from an oil-less compressor and replaced every 7 days, increasing nutrient concentration to ¼ strength in 3 weeks. Plants were then individually transferred to 2.8 L pots filled with fresh ¼ strength nutrient solution. After one week, half of the plants were transferred to fresh solution containing 250 µM Pi, while the other half was transferred to nutrient solution deprived of phosphate (Pi), with H2PO4 being replaced by H2SO4 (Muchhal et al., 1996). Roots from six plants from each treatment (0 and 250 µM Pi) were harvested 6, 12, 24 and 48 h after exposure to phosphate starvation and immediately frozen in liquid nitrogen. For each time point and each treatment, root samples were aggregated in three pools of two samples each. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of stress response

2007-05-20 | GSE4966 | GEO

Sugarcane transcriptome - ABA treatment

Project description:Plants (genotype SP80-3280, CTC, Brazil) cultivated in axenic cultures were transferred to a growth chamber at 26°C on a 16 h/8 h light/dark cycle with a photon flux density of 70 µE.m-2.s-1 and cultivated for 4 weeks. After this period, the phytohormone ABA (Sigma Chem. Co) was added to the culture medium to a final concentration of a 100 µmol.L-1. Control plants were treated with distilled water. Leaves were collected 0 h, 30 min, 1 h, 6 h and 12 h after exposure to ABA and immediately frozen in liquid nitrogen. Six plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of hormone treatment

2007-05-20 | GSE4968 | GEO

Sugarcane transcriptome - Response to N2-fixing endophytic bacteria association

Project description:Sugarcane plantlets from a variety with high inputs of N obtained from BNF (genotype SP70-1143, CTC, Brazil) free of microorganisms were obtained by sterile meristem culture and micropropagation according to the method of Hendre et al. (1983). In vitro-grown SP70-1143 rooted sugarcane plantlets were inoculated as described by James et al. (1994) with 0.1 ml of 106–107 bacterial suspension. Controls were inoculated with medium only. Endophytic diazotrophic bacteria used were Gluconacetobacter diazotrophicus (PAL5 strain) or a mixture of Herbaspirillum seropedicae (HRC54 strain) and H. rubrisubalbicans (HCC103 strain). All plants were maintained at 30°C with an irradiance of 60 µmol photons m–2 s–1 for 12 h d–1. One day after the inoculation, plant tissues were examined for bacterial colonization by the Most Probable Number (MPN) estimation, according to the methods of Reis et al. (1994) and plantlets were collected and immediately frozen in liquid nitrogen. Five plantlets were polled for each treatment. Extraction of total RNA was performed separately on each sample pool. Keywords: comparison of associations with different endophytic bacterias

2007-05-20 | GSE4970 | GEO

Sugarcane transcriptome - Drought response

Project description:Sugarcane one-eyed seed sets obtained from a variety sensitive to drought conditions (cv. SP90-1638, CTC, Brazil) were cultivated on moist sand for 15 days. The plants were transferred to pots containing moist sand and were irrigated with Hoagland’s solution (Hoagland and Arnon, 1950). Regular watering was maintained for 90 days and suppressed after this period for the experimental group. Leaves were collected 24 h, 72 h and 120 h after exposure to drought conditions for the control and experimental groups. Samples were collected and immediately frozen in liquid nitrogen. Leaves from six plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of stress response

2007-05-20 | GSE4971 | GEO

Sugarcane Expression Data from Stress Time Series

Project description:Sugarcane one-eyed seed sets obtained from a variety sensitive to drought conditions (cv. SP90-1638, CTC, Brazil) were cultivated on moist sand for 15 days. The plants were transferred to pots containing moist sand and were irrigated with Hoagland's solution (Hoagland and Arnon, 1950). Regular watering was maintained for 90 days and suppressed after this period for the experimental group. Leaves were collected 24 h, 72 h and 120 h after exposure to drought conditions for the control and experimental groups. Samples were collected and immediately frozen in liquid nitrogen. Leaves from six plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of stress response

2012-08-01 | GSE33574 | GEO

Amplification and sequencing of stress gene regions in the sugarcane cultivar SP80-3280

Project description:Complete gene regions for stress related genes in the sugarcane cultivar SP80-3280

| PRJEB43431 | ENA

Differential response to herbivory by Helicoverpa armigera in Cajanus cajan and Cajanus platycarpus

Project description:In synchrony with deciphering the transcriptomic basis of pod borer resistance in C. platycarpus, the aim of the present work was to corroborate and understand how the leaf proteome in these two contrasting cajanus species explain the differential responses to herbivory. To the best of our knowledge this is the first investigative analysis applying proteomics to understand plant herbivore interactions in cajanus species. The study is one of the best approaches to assess cellular alterations from a multi-level perception and to enhance our understanding of resistance traits in the wild relatives.

2020-07-27 | PXD017679 | Pride

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