Expression data in Arabidopsis Col-0 with cold acclimation (CA) treatment and subsequent cold deacclimation (DA) treatment.
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ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE49796 | GEO | 2014/10/02
SECONDARY ACCESSION(S): PRJNA214936
REPOSITORIES: GEO
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Project description:To understand mRNA expression pattern during cold acclimation and deacclimation, transcriptional profiling of cold acclimation and deacclimation-treated plants were analyzed using Agilent-015059 Arabidopsis 3 Oligo Microarray 4x44K G2519F. Arabidopsis Col-0 were grown on MS plate for 2 weeks (16 hours light / 8 hours dark). Two week-old Arabidopsis samples (NA, non acclimation) were treated with cold (2 M-BM-:C) for 7 days (CA7d) under 12h/12h light/dark conditions. Deacclimation-treated samples (DA6h, DA12h, DA24h) were grown at normal growth temperature under long day conditions after cold treatment for 7 days. Then total RNA was prepared from the whole seedling and used for the microarray hybridization. Three replicative hybridization experiments for each array were carried out using the independent biological samples.
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