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Gene expression analysis of prefrontal cortex in mice exposed to chronic mild stress

ABSTRACT: Major depressive disorder (MDD) is a common disorder and is responsible for considerable disability in global functioning, anorexia, and severer medical comorbidity. Recently, some reports showed the relationship between MDD and the metabolic disorders such as diabetes. We examined gene expression profiles in the mice prefrontal cortex using genome-wide microarray technology, and determined gene expression profiles with and without chronic mild stress(CMS) for 4 weeks which was often used to make models of depression. To analyze the candidate genes involved in not only depression but dysfunction of physiological homeostasis like diabetes, we campared the gene expression levels between with and without CMS, then we isolated 494 genes showing a more than 2-fold increase or a less than 1/2-fold decrease, in addition, we chose the isolated genes transcriptional products of both samples were confirmed clearly.

ORGANISM(S): Mus musculus

PROVIDER: GSE49867 | GEO | 2014/02/28

SECONDARY ACCESSION(S): PRJNA215173

REPOSITORIES: GEO

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Gene expression analysis of prefrontal cortex in mice exposed to chronic mild stress

Project description:Major depressive disorder (MDD) is a common disorder and is responsible for considerable disability in global functioning, anorexia, and severer medical comorbidity. Recently, some reports showed the relationship between MDD and the metabolic disorders such as diabetes. We examined gene expression profiles in the mice prefrontal cortex using genome-wide microarray technology, and determined gene expression profiles with and without chronic mild stress(CMS) for 4 weeks which was often used to make models of depression. To analyze the candidate genes involved in not only depression but dysfunction of physiological homeostasis like diabetes, we campared the gene expression levels between with and without CMS, then we isolated 494 genes showing a more than 2-fold increase or a less than 1/2-fold decrease, in addition, we chose the isolated genes transcriptional products of both samples were confirmed clearly. The prefrontal cortex of C57Bl/6 N sea mice with and without CMS. We mixed tatal RNA from 7 mice prefrontal cortex per each.

2014-02-28 | E-GEOD-49867 | biostudies-arrayexpress

Peripheral whole blood: Healthy control and Major depression disorder patients

Project description:Transcriptional profiling in the whole blood samples of healthy controls and major depression disorder (MDD) patients who have never been treated with depression medication. Samples included 20 healthy controls and 20 MDD patients who have never been treated with depression medication. Goal was to discover the differentially expressed genes.

2022-07-18 | GSE201332 | GEO

Genome-wide profiling of the DNA methylome in the peripheral blood of MDD patients

Project description:Genome wide DNA methylation profiling in the whole blood samples of healthy controls and major depression disorder (MDD) patients who have never been treated with depression medication. The Illumina Infinium 450k Human DNA methylation Beadchip can assay over 480K CpG sites with bisulfite-converted genomic DNA and has been widely used in methylome-wide association studies (MWAS). Samples included 40 healthy controls and 40 MDD patients who have never been treated with depression medication.

2022-07-18 | GSE201287 | GEO

ena-DATASET-KULVIB-12-11-2018-21:44:55:968-89 - samples

Project description:FGFP (Flemish Gut Flora Project, N=100) and TR-MDD (Treatment-Resistant Major Depression Disorder, N=7) shotgun sequencing samples

| EGAD00001004454 | EGA

Major depression MDD suicide brain study

Project description:Background: Major Depressive Disorder (MDD) represents a major social and economic health issue and constitutes a major risk factor for MDD suicide. The molecular pathology of suicidal depression remains poorly understood, although it has been hypothesized that regulatory genomic processes are involved in the pathology of both MDD and suicidality. Methods: Genome-wide patterns of DNA methylation were assessed in depressed MDD suicide completers (n=20) and compared to non-psychiatric, sudden-death controls (n=20) using tissue from two cortical brain regions (Brodmann Area 11 (BA11) and Brodmann Area 25 (BA25)). Analyses focussed on identifying differentially methylated regions (DMRs) associated with suicidal depression, and epigenetic variation was explored in the context of polygenic risk scores for major depression and MDD suicide. Weighted gene co-methylation network analysis was used to identify modules of co-methylated loci associated with depressed MDD suicide completers and polygenic burden for MDD and MDD suicide attempt. Results: We identified a DMR upstream of the PSORS1C3 gene, subsequently validated using bisulfite-pyrosequencing and replicated in a second set of MDD suicide samples, which is characterized by significant hypomethylation in both cortical brain regions in MDD MDD suicide cases. We also identified discrete modules of co-methylated loci associated with polygenic risk burden for MDD suicide attempt, but not major depression. MDD suicide-associated co-methylation modules were enriched among gene networks implicating biological processes relevant to depression and suicidality, including nervous system development and mitochondria function. Conclusions: Our data suggest there are coordinated changes in DNA methylation associated with MDD suicide that may offer novel insights into the molecular pathology associated with depressed MDD suicide completers.

2016-11-01 | GSE88890 | GEO

Expression data from peripheral blood lymphocytes of major depression, subsyndromal symptomatic depression, and healthy controls

Project description:Several studies have suggested that SSD is a transitory phenomena in the depression spectrum and is thus considered a subtype of depression. However, the pathophysioloy of depression remain largely obscure and studies on SSD are limited. The present study compared the expression profile and made the classification with the leukocytes by using whole-genome cRNA microarrays among drug-free first-episode subjects with SSD, MDD, and healthy controls. We used microarrays to detail the global programme of gene expression to develop blood-based gene expression profiles models for classification of subsyndromal symptomatic depression and major depressive disorder The present study compared the expression profile and made the classification with the leukocytes by using whole-genome cRNA microarrays among drug-free first-episode subjects with SSD, MDD, and matched controls (8 subjects in each group). Support vector machines (SVMs) were utilized for training and testing on candidate signature expression profiles from signature selection step.

2012-03-21 | E-GEOD-32280 | biostudies-arrayexpress

Intra-subject correlation of brain and blood gene patterns in a mouse model of depression

Project description:Gene expression profiling of blood cells in patients with major depressive disorder (MDD) has been used to identify potential biomarkers and to address the pathophysiology of MDD. However, whether alteration in gene expression in blood cells are reflected in the brain of the same individual is unclear. Here, we used an animal model of depression to investigate intra-subject correlation of gene expression patterns between the whole blood (WB) and the medial prefrontal cortex (mPFC). Ovariectomized mice exposed to the chronic mild stress were used as an animal model of depression.

2015-08-21 | GSE72262 | GEO

Multi-brain-region transcriptional organization linking sleep and affective functions

Project description:Sleep and affective behaviors are highly interrelated phenotypes, commonly altered in a variety of neuropsychiatric diseases, including major depressive disorder (MDD). To understand the transcriptomic organization underlying sleep and affective function, we studied a population of (C57BL/6J x 129S1/SvImJ) F2 mice by measuring 283 affective and sleep phenotypes and profiling gene expression across four brain regions, including the frontal cortex, hippocampus, thalamus, and hypothalamus. We identified converging molecular bases for sleep and affective phenotypes at both the single-gene and gene-network levels. Utilizing publicly available transcriptomic datasets collected from sleep-deprived mice and major depressive disorder (MDD) patients, we identified three cortical gene networks altered by sleep/wake changes and depression. The network-level actions of sleep loss and depression were opposite to each other, providing a mechanistic basis for the sleep disruptions commonly observed in depression as well as the reported acute antidepressant effects of sleep deprivation. We highlight one particular network composed of circadian rhythm regulators and neuronal activity-dependent immediate-early genes. The key upstream driver of this network, Arc, may act as a nexus linking sleep and depression. Our data provide mechanistic insights into the role of sleep in affective function and MDD.

2018-12-21 | GSE109112 | GEO

Circuit-wide Proteomics Profiling Reveals Brain Region-specific Protein Signatures in the WKY Rats with Endogenous Depression

Project description:Major depressive disorder (MDD) is considered as a neural circuit-based polygene syndrome that is mainly triggered by genetic susceptibility and stress factors. The present study employed the Wistar Kyoto (WKY) rat as an animal model with endogenous depression to further investigate the molecular basis of its genetic susceptibility to depression by performing quantitative protemoics analyses of the medial prefrontal cortex (mPFC), nucleus accumbens (NAc), and hippocampus (Hip), respectively.

2022-10-13 | PXD029079 | Pride

Gene expression alteration in the hippocampus of depression mouse model with forced swim stress

Project description:We used microarrays to identify the gene with altered expression in the hippocampus of depression mouse model with forced swim stress campared to control mice .

2023-02-17 | GSE223430 | GEO

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