Identification of Smaug target mRNAs in the early Drosophila embryo using RIP-Chip

ABSTRACT: To identify Smaug’s target mRNAs on a genome-wide scale we used ribonucleoprotein (RNP) co-immunoprecipitation followed by microarray analysis of the co-purifying mRNAs (RIP-Chip). Extracts, prepared from wild-type embryos collected 0-3 hours post-egglaying, were immunoprecipitated with an anti-Smaug antibody (Smaug RIPs) while control immunoprecipitations using non-immune serum served as a negative control (control RIPs). Genome-wide transcript expression in wild-type embryos were also assessed and used as reference.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE49943 | GEO | 2013/12/03

SECONDARY ACCESSION(S): PRJNA215420

REPOSITORIES: GEO