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Runx3 function in splenic NK cells activated in vivo by IL-15.

ABSTRACT: NK cells are innate immune cells that recognize and kill foreign, virally-infected and tumor cells without the need for prior immunization. NK expansion following viral infection is IL-2 or IL-15-dependent.

ORGANISM(S): Mus musculus

PROVIDER: GSE50120 | GEO | 2014/01/22

SECONDARY ACCESSION(S): PRJNA216118

REPOSITORIES: GEO

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Runx3 function in splenic NK cells (IL-2 or IL-15)

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Runx3 function in splenic NK cells (IL-2 or IL-15)

Project description:NK cells are innate immune cells that recognize and kill foreign, virally-infected and tumor cells without the need for prior immunization. NK expansion following viral infection is IL-2 or IL-15-dependent. To identify Runx3 responsive genes, NK cells were isolated from spleen of WT and Runx3-/- mice . Ten samples (5 WT and 5 Runx3-/-) of freshly isolated NK cells were separately obtained from individual mice. Cells were cultured for 7 days with IL-2 or IL-15.

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2014-01-22 | E-GEOD-50120 | biostudies-arrayexpress

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Genome-wide maps of Runx3 bound regions in splenic NK cells activated in vivo with IL-15

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2014-01-22 | E-GEOD-50126 | biostudies-arrayexpress

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Project description:ChIP-seq was conducted using splenic WT NK cells cultured for 7 days with IL-2 using anti-Runx3 antibodies (Ab), anti-H3K4me1 Ab and non-immune serum (NIS) as control. Two biological Runx3, one H3K4me1 and two NIS IP repeats from splenic NK cells isolated from individual mice by negative selection using NK cell isolation kit (R&D) cultured with IL-2.

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NK-cell derived extracellular vesicles LC-MS/MS

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CIS is a potent checkpoint in NK cell-mediated tumour immunity

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