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Transcriptional Response of Mycobacterium tuberculosis CDC1551 Rv0516c::Tn to treatment with 140 mM NaCl for 1 hr

ABSTRACT: To characterize the role of Rv0516c in the osmotic stress response of Mycobacterium tuberculosis (Mtb), we performed transcriptional profiling of CDC1551 Rv0516c::Tn following treatment with 140 mM NaCl for 1 hr relative to an untreated control.

ORGANISM(S): Mycobacterium tuberculosis

PROVIDER: GSE50160 | GEO | 2013/11/15

SECONDARY ACCESSION(S): PRJNA217031

REPOSITORIES: GEO

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Transcriptional Response of Mycobacterium tuberculosis CDC1551 Rv0516c::Tn to treatment with 140 mM NaCl for 1 hr

Project description:To characterize the role of Rv0516c in the osmotic stress response of Mycobacterium tuberculosis (Mtb), we performed transcriptional profiling of CDC1551 Rv0516c::Tn following treatment with 140 mM NaCl for 1 hr relative to an untreated control. CDC1551 Rv0516c::Tn was treated with 140 mM NaCl for 1 h and compared to the same strain treated with 0 mM NaCl for 0 h. 4 biological replicates, independently grown and harvested. One replicate per array.

2013-11-15 | E-GEOD-50160 | biostudies-arrayexpress

Transcriptional Response of Mycobacterium tuberculosis CDC1551 to treatment with 140 mM NaCl for 1 hr

2013-11-15 | GSE50159 | GEO

Transcriptional Response of Mycobacterium tuberculosis CDC1551 to treatment with 140 mM NaCl for 1 hr

Project description:To identify mediators of the osmotic stress response in Mycobacterium tuberculosis (Mtb), we performed transcriptional profiling of WT CDC1551 following treatment with 140 mM NaCl for 1 hr relative to an untreated control. 140 mM NaCl was chosen to reflect the approximate osmolarity of human plasma (i.e., 280 mOsm/L), which may be relevant during the course of infection. CDC1551 was treated with 140 mM NaCl for 1 h and compared to the same strain treated with 0 mM NaCl for 0 h. 4 biological replicates, independently grown and harvested. One replicate per array.

2013-11-15 | E-GEOD-50159 | biostudies-arrayexpress

Effect of NaCl on CDC1551 gene expression

Project description:Gene Expression profiling of M. tuberculosis CDC1551 treated with 0.25 M NaCl vs untreated samples. The dataset includes 5 biological replicates.

2013-04-05 | E-MTAB-1374 | biostudies-arrayexpress

Mycobacterium tuberculosis

Project description:Transcriptional Response of Mycobacterium tuberculosis CDC1551 Rv0516c::Tn to treatment with 140 mM NaCl for 1 hr

| PRJNA217031 | ENA

EXoO-Tn: Tag-n-Map the Tn Antigen in the Human Proteome

Project description:Tn antigen (Tn), a single N-acetylgalactosamine (GalNAc) monosaccharide attached to protein Ser and Thr residues, is found on most solid tumors yet rarely detected in adult tissues: featuring it one of the most distinctive signatures of cancers. Although it is prevalent in cancers, Tn-glycosylation sites are not entirely clear owing to the lack of suitable technology. Knowing the Tn-glycosylation sites will spur the development of the new vaccines, diagnostics, and therapeutics of cancers. Here, we report a novel technology named EXoO-Tn for large-scale mapping of Tn-glycosylation sites. EXoO-Tn utilizes glycosyltransferase C1GalT1 and isotopically-labeled UDP-Gal(13C6) to tag and convert Tn to Gal(13C6)-Tn. This exquisite Gal(13C6)-Tn structure is recognized by a human-gut-bacterial enzyme, called OpeRATOR, that specifically cleaves N-termini of the Gal(13C6)-Tn-occupied Ser and Thr residues to yield site-containing glycopeptides. The enzymes C1GalT1 and OpeRATOR could be used concurrently in one-pot. The effectiveness of EXoO-Tn was evaluated by analyzing Jurkat cells, where 947 Tn-glycosylation sites from 480 glycoproteins were mapped. Bioinformatic analysis of the identified site-specific Tn-glycoproteins revealed conserved motif, cellular localization, relative position in proteins, and mapped site-specific Tn-glycoproteome in different studies. Given the significance of Tn in cancers, EXoO-Tn is anticipated to have broad utilities in clinical study of cancers.

2022-02-15 | PXD014390 | Pride

Mycobacterium tuberculosis CDC1551

Project description:Mycobacterium tuberculosis CDC1551 WT/sigI mutant

| PRJNA152857 | ENA

NaCl reactors

Project description:NaCl reactors Metagenome

| PRJNA690829 | ENA

Expression data in an Arabidopsis cytokinin (CK)-signaling mutant, ahp2,3,5 and arr1,10,12, and its wild type plant (WT, Col-0) under high salinity (200 mM NaCl) and control (0 mM NaCl) conditions.

Project description:To understand the role of CK-signaling components, AHPs (His-containing phosphotransfer proteins) and type-B ARRs (response regulators) in salt stress response, we have employed transcriptional profiling of ahp2,3,5 and arr1,10,12, and it's wild type plant (WT), Col-0 under high salinity (200 mM NaCl) and control (0 mM NaCl) conditions. Agilent’s Whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used.

2017-12-31 | GSE94885 | GEO

Light dependent gene expression in D. shibae wildtype compared to the gene expression in the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn

Project description:Light dependent gene expression in D. shibae wildtype compared to the gene expression in the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn. Dinoroseobacter shibae DFL12T (DSM 16493T) and the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn were grown in artificial saltwater minimal medium (SWM) in baffled flasks shaking at 180 rpm and 30 °C and incubation was performed under light, dark and bluelight conditions. D. shibae wild type and mutant strain were grown under aerobic conditions up to the mid exponential growth phase (OD578 nM 0.5).

2023-10-01 | GSE244289 | GEO

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