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Inducible overexpression analysis of KUODA1 in Arabidopsis seedlings

ABSTRACT: To identify potenital target genes of the transcription factor KUODA1 (AT5G47390), we performed expression profiling using an estradiol inducible overexpression system.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE50520 | GEO | 2013/12/31

SECONDARY ACCESSION(S): PRJNA217830

REPOSITORIES: GEO

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Inducible overexpression analysis of KUODA1 in Arabidopsis seedlings

Project description:To identify potenital target genes of the transcription factor KUODA1 (AT5G47390), we performed expression profiling using an estradiol inducible overexpression system. Estradiol-treatment allows for the nuclear translocation of the constitutively expressed chimeric transcription factor XVE and subsequent expression of KUA1 from the LexA promoter. Stable trangenic T3 seedlings were grown for 14 days and either treated with 15 M-BM-5M estradiol or ethanol (mock) for 4 hours. Two mock and two estradiol treated samples were used for RNA isolation and hybridization.

2013-12-31 | E-GEOD-50520 | biostudies-arrayexpress

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2013-01-31 | E-GEOD-38878 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:Identification of ORB1 target genes using inducible overexpression system

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Project description:Identification of ORS1 target genes using inducible overexpression system

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Project description:The aim was to identify genes associated to the down-regulation TARGET OF RAPAMYCIN (TOR) pathway in Arabidopsis. For this purpose, three independent amiR-tor lines (amiR-tor9, amiR-tor17 and amiR-tor 20) and EV lines 3 and 6 days after EST- or non-induction were used for expression profile analysis using Affymetrix microarray. Two weeks-old transgenic seedlings were transferred to MS plate with 20µM estradiol to induce the overexpression of amiR-tor (consequently, repression of AtTOR levels) under the control of an estradiol-(EST) inducible promoter. Identically treated wild-type Col-0 and pER8 empty-vector (EV) transformed seedlings were served as controls. Seedlings were harvested after 3 and 6 (amiR-tor lines) days.

2013-01-31 | GSE38878 | GEO

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