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RNA-Seq analysis of KLF3 knockout E14.5 TER119+ (erythroid) murine fetal liver cells

ABSTRACT: The aim of this study was to analyze the transcriptome of TER119+ fetal liver cells in the absence of the transcription factor KLF3 at murine embryonic day E14.5

ORGANISM(S): Mus musculus

PROVIDER: GSE50554 | GEO | 2014/02/23

SECONDARY ACCESSION(S): PRJNA217929

REPOSITORIES: GEO

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RNA-Seq analysis of KLF3 knockout E14.5 TER119+ (erythroid) murine fetal liver cells

Project description:The aim of this study was to analyze the transcriptome of TER119+ fetal liver cells in the absence of the transcription factor KLF3 at murine embryonic day E14.5 Three wildtype (WT; Klf3+/+) and three knockout (KO; Klf3-/-) samples

2014-02-23 | E-GEOD-50554 | biostudies-arrayexpress

Microarray analysis of wildtype and Klf3 KO E14.5 fetal liver cells (TER119- and TER119+)

Project description:The aim of this experiment was to investigate the role of KLF3 in regulating gene expression at different stages throughout the erythroid maturation process. Affymetrix microarrays were performed on fetal liver cells (both TER119- progenitor cells and TER119+ erythroblast cells) from E14.5 wildtype and Klf3 KO mice. Four wildtype TER119- replicates, four Klf3 KO TER119- replicates, four wildtype TER119+ replicates, three Klf3 KO TER119+ replicates. All are from E14.5 fetal liver.

2012-02-07 | E-GEOD-35592 | biostudies-arrayexpress

Microarray analysis of wildtype and Klf3 KO E14.5 fetal liver cells (TER119- and TER119+)

2012-02-07 | GSE35592 | GEO

Microarray analysis of wildtype, Klf8gt/gt, Klf3-/- and Klf3-/- Klf8gt/gt TER119+ E13.5 fetal liver cells

2013-01-17 | GSE43524 | GEO

Microarray analysis of wildtype, Klf8gt/gt, Klf3-/- and Klf3-/- Klf8gt/gt TER119+ E13.5 fetal liver cells

Project description:The aim of this experiment was to investigate the regulation of gene expression by KLF3 and KLF8 in fetal erythroid cells by analyzing single and double mutant mouse models. Affymetrix microarrays were performed on RNA from TER119+ fetal liver cells from E13.5 wildtype, Klf8gt/gt, Klf3-/- and Klf3-/- Klf8gt/gt mice. Four wildtype replicates, four Klf8gt/gt replicates, three Klf3-/- replicates and four Klf3-/- Klf8gt/gt replicates of E13.5 TER119+ fetal liver cell samples, litter-matched where possible.

2013-01-17 | E-GEOD-43524 | biostudies-arrayexpress

RNA-Seq analysis of microRNA expression profiles in mouse primary CFU-E late erythroid progenitors and Ter119+ mature erythroblasts

Project description:Using RNA-seq technology, we quantitatively determined the expression profile of microRNAs during mouse terminal erythroid differentiation. CFU-E erythroid progenitors were isolated from E14.5 fetal liver as the Ter119, B220, Mac-1, CD3 and Gr-1 negative, C-Kit positive and 20% high CD71 population. Mature Ter119+ erythroblasts were isolated from E14.5 fetal liver as C-Kit negative and Ter119 positive population. Consistent with nuclear condensation and global gene expression shut down during terminal erythroid differentiation, we found that the majority of microRNAs are downregulated in more mature Ter119+ erythroblasts compared with CFU-E erythroid progenitors.

2011-06-09 | GSE25602 | GEO

RNA-Seq analysis of microRNA expression profiles in mouse primary CFU-E late erythroid progenitors and Ter119+ mature erythroblasts

2011-06-09 | E-GEOD-25602 | biostudies-arrayexpress

Mus musculus

Project description:Microarray analysis of wildtype and Klf3 KO E14.5 fetal liver cells (TER119- and TER119+)

| PRJNA152505 | ENA

Comprehensive expression profiling across primary fetal liver terminal erythroid differentiation

Project description:Primary murine fetal liver cells were freshly isolated from day e14.5 livers and then sorted for successive differentiation stages by Ter119 and CD71 surface expression (ranging from double-negative CFU-Es to Ter-119 positive enucleated erythrocytes) [Zhang, et al. Blood. 2003 Dec 1; 102(12):3938-46]. RNA isolated from each freshly isolated, stage-sorted population was reverse-transcribed, labelled, and then hybridized onto 3' oligo Affymetrix arrays. Important erythroid specific genes as well as the proteins that regulate them were elucidated through this profiling based on coexpression and differential expression patterns as well as by extracting specific GO categories of genes (such as DNA-binding proteins).

2010-06-16 | GSE20391 | GEO

Transcription profiling of Cited2 knockout mouse E14.5 fetal liver samples

Project description:Total RNA (15 ug) isolated from three each of wild type and Cited2-null E14.5 fetal livers using Qiagen RNeasy kit (Valencia, CA) was used to prepare biotinylated cRNA according to the protocol described in Affymetrix Expression Analysis Technical Manual. We hybridized a total of six GeneChip Mouse Genome 430A 2.0 arrays (Affymetrix), three for controls and three for experimental samples.

2007-09-11 | E-MEXP-1240 | biostudies-arrayexpress

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