Transcriptomics,Genomics

Dataset Information

46

Airway epithelium, small airways, normal non-smokers, phenotypic normal smokers, smokers with COPD and early COPD


ABSTRACT: Upregulation of Expression of the Ubiquitin Carboxyl Terminal Hydrolase L1 Gene in Human Airway Epithelium of Cigarette Smokers The microarray data deposited here is from 39 HG-U133 Plus 2.0 GeneChips, from 12 normal non-smokers, 12 phenotypic normal smokers, 9 Early COPD and 6 COPD individuals, all small airways, all small airway. A subset of these samples have been already submitted under GEO Accession Number GSE 4498. These are: 12 non-smokers samples (GSM101095-GSM101106) and 10 smoker samples (GSM101107-GSM101116). These 22 samples that are also in GSE4498 were described in Harvey, B-G; Heguy, A.; Leopold, P.L.; Carolan, B.; Ferris, B. and Crystal R.G. Modification of Gene Expression of the Small Airway Epithelium in Response to Cigarette Smoking. J. Mol. Med (in press). These data are part of a study aimed at understanding how cigarette smoking modifies neuroendocrine cells, in which microarray analysis with TaqMan confirmation was used to assess airway epithelial samples obtained by fiberoptic bronchoscopy from 81 individuals (normal nonsmokers, normal smokers, smokers with early COPD and smokers with established COPD). Of 11 genes considered to be neuroendocrine cell-specific, only ubiquitin C-terminal hydrolase L1(UCHL1), a member of the ubiquitin proteasome pathway, was consistently upregulated in smokers compared to nonsmokers. Up-regulation of UCHL1 at the protein level was observed with immunohistochemistry of bronchial biopsies of smokers compared to nonsmokers. Interestingly, however, while UCHL1 expression was present only in neuroendocrine cells of the airway epithelium in nonsmokers, UCHL1 expression was also expressed in ciliated epithelial cells in smokers, an intriguing observation in light of recent observations that ciliated cells can are capable of transdifferentiating to other airway epithelium. In the context that UCHL1 is involved in the degradation of unwanted, misfolded or damaged proteins within the cell and is overexpressed in >50% of lung cancers, its overexpression in chronic smokers may represent an early event in the complex transformation from normal epithelium to overt malignancy. Keywords: non-smokers vs phenotypic normal smokers, smokers with early COPD, and smokers with COPD Overall design: comparison of gene expression in airway epithelial cells of the small airways of normal non-smokers, phenotypic normal smokers, smokers with early COPD and smokers with COPD

REANALYSED by: GSE60486GSE119128

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Yael Strulovici-Barel  

PROVIDER: GSE5058 | GEO | 2006-11-21

SECONDARY ACCESSION(S): PRJNA104499

REPOSITORIES: GEO

altmetric image

Publications

Up-regulation of expression of the ubiquitin carboxyl-terminal hydrolase L1 gene in human airway epithelium of cigarette smokers.

Carolan Brendan J BJ   Heguy Adriana A   Harvey Ben-Gary BG   Leopold Philip L PL   Ferris Barbara B   Crystal Ronald G RG  

Cancer research 20061101 22


Neuroendocrine differentiation is a common feature of lung cancer and increased numbers of neuroendocrine cells and their peptides have been described in chronic smokers. To understand the effects of cigarette smoking on the gene expression profile of neuroendocrine cells, microarray analysis with TaqMan confirmation was used to assess airway epithelial samples obtained by fiberoptic bronchoscopy from 81 individuals [normal nonsmokers, normal smokers, smokers with early chronic obstructive lung  ...[more]

Similar Datasets

2009-04-25 | E-GEOD-5057 | ArrayExpress
2008-11-07 | E-GEOD-5056 | ArrayExpress
2008-12-19 | GSE10006 | GEO
2008-12-19 | E-GEOD-10006 | ArrayExpress
2008-12-16 | GSE7832 | GEO
2006-11-21 | GSE4498 | GEO
2009-01-13 | E-GEOD-7832 | ArrayExpress
2005-10-30 | GSE3320 | GEO
2012-10-03 | E-GEOD-30063 | ArrayExpress
2008-06-17 | GSE8545 | GEO