Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression of SUM159 breast cancer cell line expressing microRNA--203

ABSTRACT: Determine the effect of miR-203 expression on the global mRNA expression in mesenchymal breast cancer cell line.

ORGANISM(S): Homo sapiens

PROVIDER: GSE50697 | GEO | 2013/09/23

SECONDARY ACCESSION(S): PRJNA218516

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression of SUM159 breast cancer cell line expressing microRNA--203

Project description:Determine the effect of miR-203 expression on the global mRNA expression in mesenchymal breast cancer cell line. Generate control (pBabe puro) and miR-203 (pBabe puro miR-203) cells using retroviral transduction and puromycin selections. Extract total RNA using Trizol.

2013-09-23 | E-GEOD-50697 | biostudies-arrayexpress

Gene expression of SUM159 breast cancer cell line expressing microRNA--203

Project description: Not available

| S-ECPF-GEOD-50697 | biostudies-other

Genes regulated by miR-203 in breast cancer cell line MDA-MB-231

2013-05-01 | GSE44239 | GEO

Genes regulated by miR-203 in breast cancer cell line MDA-MB-231

Project description:To investigate the mechanism through which miR-203 inhibited the breast cancer cell invasion, we overpression miR-203 in MDA-MB-231 cell line and performed a microarray to examine the genes which maybe targeted and down-regulated by miR-203. We made a stable cell line(MB231/miR-203) which overexpressing miR-203 and a control cell line (MB231/EV) which expressing a contrlol vector. Total RNA were extracted from these two cell lines and subject to microarray.

2013-05-01 | E-GEOD-44239 | biostudies-arrayexpress

cAMP-MicroRNA-203-IFNγ Network Regulates Subcutaneous White Fat Browning and Glucose Tolerance

Project description:To determine the function of miR-203 in white fat browning upon cold exposure, we injected miR-203 inhibitors and negative control into inguinal white adipose tissue, followed by cold exposure (4oC) for 24 hours. Total RNA were harvested for RNA-seq.

2022-06-21 | GSE133142 | GEO

Expression data of mRNA from wild-type A549 cells and microRNA-203 knockout A549 cells during H5N1 infections

Project description:MicroRNA-203 was up-regulated markedly upon H5N1 virus infection. To identify the potential target genes of miR-203, we constructed a miR-203 knockout A549 cell line. Then wild-type and miR-203 knockout A549 cells were mock-infected or infected with H5N1 virus for 48h. The Agilent Whole Human Genome Oligo Microarray was performed to analyze the mRNA expression profiling. Meanwhile, the online tool TargetScanHuman (http://www.targetscan.org/vert_71/) was used to predict biological targets of miR-203. We combined the predicted genes with the genes differentially expressed in wild-type and miR-203 knockout A549 cells, and preliminarily identified some candidate mRNAs. Then more experiments were performed to further verify these target genes, such as dual-luciferase reporter assay, quantitative real-time PCR or Western blot analysis.

2018-05-09 | GSE107089 | GEO

miR-203, a microRNA of multilayered epithelia, inhibits metastatic fitness in human squamous cell carcinoma

Project description:The colonization of distant organs by metastatic carcinoma cells underpins most human cancer-related deaths, including those from head and neck squamous cell carcinoma (HNSCC). We report that miR-203, a miRNA that promotes keratinocyte differentiation, is necessary and sufficient to inhibit multiple post-extravasation events during HNSCC lung metastasis, including initial survival/engraftment, escape from metastatic dormancy, and overt colonization in vivo. Restoration of miR-203 expression in established lung metastases reduces overall metastatic burden. Instead of promoting differentiation, miR-203 controls lung metastasis through direct targeting of genes involved in cytoskeletal dynamics (LASP1), ECM remodeling (SPARC), and cell metabolism (NUAK1). Expression of miR-203 and its downstream targets correlates with HNSCC overall survival outcomes, suggesting the therapeutic potential of targeting this signaling axis.

2016-01-01 | GSE47028 | GEO

Expression data from miR-203 induction in mouse skin

Project description:We identify numerous miR-203 in vivo targets that are highly enriched for the promotion of cell cycle and cell division. Importantly, individual targets including p63, Skp2 and Msi2 play distinct roles downstream of miR-203 to regulate the cell cycle and long-term proliferation. Together, our findings reveal rapid and widespread impact of miR-203 on the self-renewal program during the epidermal differentiation and provide mechanistic insights for the potent role of miR-203 where coordinated repression of multiple targets is required for the function of this miRNA. We used microarrays to measure transcriptome changes upon miR-203's induction in mouse skin and identified new targets of miR-203.

2013-03-14 | GSE45121 | GEO

miR-203, a microRNA of multilayered epithelia, inhibits metastatic fitness in human squamous cell carcinoma

2016-07-03 | E-GEOD-47028 | biostudies-arrayexpress

Gene expression analysis in islets from miR-203 TG mice [scRNA-seq]

Project description:To investigate the effect of miR-203 in type 2 diabetes, target genes of miR-203 need to be investigated. The β cell specific miR-203 transgene (miR-203 TG) mice was constructed, and scRNA-seq was then performed on mouse islets.

2023-05-19 | GSE232474 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data