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Role of de novo DNA methyltransferases in hematopoietic stem cells (RNA-seq)

ABSTRACT: To investigate the global transcriptome expression in Dnmt3ab dKO HSCs, we performed high-throughput sequencing of Poly A+ RNA (RNA-Seq) from purified HSCs (SP-KSL-CD150+). With biological duplicates, more than 200 million reads in total for each genotype of HSC were obtained.

ORGANISM(S): Mus musculus

PROVIDER: GSE50775 | GEO | 2014/08/19

SECONDARY ACCESSION(S): PRJNA218934

REPOSITORIES: GEO

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Role of de novo DNA methyltransferases in hematopoietic stem cells (RNA-seq)

Project description:To investigate the global transcriptome expression in Dnmt3ab dKO HSCs, we performed high-throughput sequencing of Poly A+ RNA (RNA-Seq) from purified HSCs (SP-KSL-CD150+). With biological duplicates, more than 200 million reads in total for each genotype of HSC were obtained. Mouse hematopoietic stem cell mRNA profiles of Dnmt3ab Dko HSCs, generated by deep sequencing, in duplicate, using Illumina Hiseq 2000

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