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Saos-2 vs. Saos-2-2 cells

ABSTRACT: Saos-2-2, a TSG101-deficient stable line, was dereived from Saos-2 cells. The goal of this experiment was to determine the effects of TSG101 down-regulation on global gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE50808 | GEO | 2013/10/11

SECONDARY ACCESSION(S): PRJNA219061

REPOSITORIES: GEO

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Saos-2 vs. Saos-2-2 cells

Project description:Saos-2-2, a TSG101-deficient stable line, was dereived from Saos-2 cells. The goal of this experiment was to determine the effects of TSG101 down-regulation on global gene expression. Saoa-2 and Saos-2-2 samples were labeled with Cys-3 and Cys-5 respectively, and hybrided with microarray simutaneously to differentiate the up- and down-regulated genes.

2013-10-11 | E-GEOD-50808 | biostudies-arrayexpress

SAOS-2 cells, RBP2 knockdown or pRB overexpression

Project description:RBP2 is downstream of pRB pathway We used gene expression profiling experiments to investigate if gene expression changes in cells with RBP2 knockdown significantly overlap with gene expression changes in cells overexpressing pRB, consistent with the data that knockdown of RBP2 phenocopies reintroduction of pRB in SAOS-2 (RB-/-) cells Keywords: epistatic experiment

2008-08-29 | GSE9690 | GEO

In vitro biocompatibility of ZrO2 vs Y-TZP alloys on Saos-2 cells: influence of composition and surface topography

Project description:This study assess the biocompatibility of ZrO2 vs Y-TZP alloys on Saos-2 cells, through the combination of conventional toxicological assay, morphological observations and transcriptomic analysis performed on Saos-2 cells. The biocompatibility of ZrO2 alloys was evaluated in comparison with Y-TZP alloys, studying cell proliferation (WST-1) and cell morphology/adhesion (SEM) on human Saos-2 cell lines. Finally, an evaluation of gene expression was performed on Y-TZP alloys rough surface that seems more interesting regarding biocompatibility results.

2022-07-13 | GSE203572 | GEO

The effects of SMYD2 overexpression on ectopic RB in SAOS cells

Project description:Expression analysis of wild-type SAOS cells and SAOS cells transiently transfected with RB, SMYD2, or RB and SMYD2.

2011-01-01 | GSE22842 | GEO

Identification of target genes regulated by ΔNp63α in SaOS-2 cells stably expressed with ΔNp63α

Project description:To identify the target gene of ΔNp63α, in osteosarcoma cells, we performed the global gene expression profiling in SaOS- 2 cells stably overexpressing the ΔNp63α gene (SaOS- 2-ΔNp63α). ΔNp63α target genes were identified by comparison with genes expressed from SaOS-2-EV transfected with an empty vector ( SaOS-2-EV).

2014-02-27 | GSE54942 | GEO

DNase-seq profiling of Saos-2 and U2OS cell lines

Project description:We performed DNase-seq on two osteosarcoma cell lines, Saos-2 and U2OS. This was done to identify regulatory regions within the genomes of these cells. We performed hotspot calling on these data using Hotspot2 (https://github.com/Altius/hotspot2) to identify 51,556 DNaseI hypersensitive sites within Saos-2 cells and 63,388 within U2OS cells.

2020-01-03 | GSE142160 | GEO

Effects of PRIMA-1 treatment on Saos-2 p53-null and Saos-2 p53-His273 cells

Project description:This SuperSeries is composed of the following subset Series: GSE15367: Expression analysis of Saos-2 p53-null cells GSE15368: Expression analysis of Saos-2 cells expressing p53-His273 Refer to individual Series

2009-04-15 | E-GEOD-15658 | biostudies-arrayexpress

In vitro biocompatibility of Ti-Cp vs Ti-26Nb alloys on Saos-2 and THP-1 cells

Project description:This study assess the biocompatibility of Ti-26Nb compared to Ti-Cp, a reference in implantology, through the combination of conventional toxicological assay, morphological observations and transcriptomic analysis performed on two different cells line : Saos-2 and THP-1. The biocompatibility of Ti-Cp implants was evaluated in comparison with Ti-26Nb implants both having a mirror polished surface, studying cell proliferation (trypan blue and WST-1) and cell morphology/adhesion (SEM) on human THP-1 and Saos-2 cell lines. Finally, an evaluation of a potential toxicity of potassium niobiate powder KNbO3 was carried out by measuring metabolic activity and realizing a transcriptomic study on the THP-1 line (the first one) to deepen the results observed with Ti-26Nb discs. Indeed, niobiate ions are potentially released from implant in cellular acidic environnement. Concerning Saos-2 cells, our results suggest that Ti-26Nb and Ti-Cp discs do not impact proliferation and viability. Concerning THP-1 cell line, a decrease in proliferation and viability is observed in contact of Ti-26Nb discs (hypothesis of a cell-line effect ?, no biocompatibility study on monocyte/macrophage cell lines such as THP-1 actually available in literature). KNbO3 powder does not impact viability of THP-1 cells and does not induce changes at molecular level. Taken together, these data suggest a possible toxicity of Ti-26Nb toward THP-1 cells not directly related to the niobium but perhaps to the manufacturing process of Titane niobium alloy.

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RNAi profiling of human SAOS-2 cells, RBP2 knockdown or pRB overexpression

Project description:RBP2 is downstream of pRB pathway; We used gene expression profiling experiments to investigate if gene expression changes in cells with RBP2 knockdown significantly overlap with gene expression changes in cells overexpressing pRB, consistent with the data that knockdown of RBP2 phenocopies reintroduction of pRB in SAOS-2 (RB-/-) cells Experiment Overall Design: Knockdown of RBP2 by siRNA to RBP2 (RBP2siRNA) in SAOS-2 (RB-/-) cells in comparison to overexpression of pRB in the SOAS-2 (RB-/-) cells and scrambled version of the RBP2 siRNA (RBP2scsiRNA)

2008-10-25 | E-GEOD-9690 | biostudies-arrayexpress

Bacillaceae bacterium SAOS 7

Project description:Indiibacillus sp. (SAOS 7) Genome sequencing and assembly

| PRJNA390294 | ENA