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Global microarray analysis of changes of gene expression in the epidermis of Brg1(i)ep-/- mice at E16.5

ABSTRACT: Changes in global gene expression in the epidermis of the Brg1(i)ep-/- mice in comparison to the wild type at E16.5 were analyzed using micro-array technology.

ORGANISM(S): Mus musculus

PROVIDER: GSE50847 | GEO | 2013/11/01

SECONDARY ACCESSION(S): PRJNA219162

REPOSITORIES: GEO

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Global microarray analysis of changes of gene expression in the epidermis of Brg1(i)ep-/- mice at E16.5

Project description:Changes in global gene expression in the epidermis of the Brg1(i)ep-/- mice in comparison to the wild type at E16.5 were analyzed using micro-array technology. The RNA was purified from the mouse epidermal tissue after laser capture microdisection (LCM) followed by two rounds of linear amplification. The amplified RNA from two Brg1(i)ep-/- animals were pooled together and the amplified RNA from two control animals was also were pooled together.

2013-11-01 | E-GEOD-50847 | biostudies-arrayexpress

EP - GMP

Project description:Mouse erythroid progenitors (EP) in comparison to granulocyte/monocyte - macrophage progenitors (GMP) from 10 - 16 week old C57/Bl6 - S129Ola (mixed genetic background) purified by flow cytometry Keywords = EP Keywords = GMP Keywords = hematopoiesis Keywords: other

2004-10-22 | GSE1584 | GEO

The core SWI/SNF catalytic subunit Brg1 regulates nephron progenitor cell proliferation and differentiation [ChIP-seq]

Project description:Binding of Brg1, Sall1, H3K4me1, H3K27ac, and RNA Pol2 Ser2 to chromatin was measured by performing ChIP-seq in E16.5 wild type kidney.

2022-02-19 | GSE196995 | GEO

Protein-protein interactions of BRG1 or BRG1-HSA in SW13 cells

Project description:The SWI/SNF complex remodels chromatin in an ATP-dependent manner through the ATPase subunits BRG1 and BRM. Chromatin remodeling alters nucleosome structure to change gene expression, however aberrant remodeling and gene expression can result in cancer. The function and localization on chromatin of the SWI/SNF complex depends on the protein makeup of the complex. Here we report the protein-protein interactions of wild-type BRG1 or mutant BRG1 in which the HSA domain has been deleted (BRG1-HSA). We demonstrate the interaction of BRG1 with most SWI/SNF complex members and a failure of a number of these members to interact with BRG1-HSA. These results demonstrate that the HSA domain of BRG1 is a critical interaction platform for the correct formation of SWI/SNF remodeling complexes.

2023-02-15 | PXD029647 | Pride

Mus musculus

Project description:Global microarray analysis of changes of gene expression in the epidermis of Brg1(i)ep-/- mice at E16.5

| PRJNA219162 | ENA

RNA-Seq of epidermis and mesophyll in arabidopsis developing embryos

Project description:ngs2014_01_microd-ngs micro d-1-RNA seq of epidermis and mesophyll in arabidopsis developing embryos-comparison of transcript accumulation in epidermis and mesophyll in Total RNA extracted by laser assisted microdissection from developping embryos at late heart stage.

2018-02-13 | GSE98176 | GEO

Transcriptome analysis the genes expression in SsMYRV4-infected strain Ep-1PNA367T1 and SsMYRV4-free strain Ep-1PNA367

Project description:Transcriptome analysis the genes expression in SsMYRV4-infected strain Ep-1PNA367T1 and SsMYRV4-free strain Ep-1PNA367. the two strains were grown on a cellophane membrane overlaid onto PDA medium at 20℃ for 3 days, samples were collected for total RNA extraction. The RNA samples were sequenced and anlysized by the BGI Co., Ltd. Total clean reads of each sample above 5000000.The sequence reads that passed quality filters were analyzed at the transcript isoform level with TopHat followed by Cufflinks.

2017-02-07 | GSE94575 | GEO

Sclerotinia sclerotiorum strain:Ep-1PNA367T1 and Ep-1PNA367

Project description:Sclerotinia sclerotiorum strain:Ep-1PNA367T1 and Ep-1PNA367 Transcriptome sequencing

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RNA-seq of SCLC PDX models treated with EP

Project description:We report the correlation of transcript abundance with response to EP in 19 PDX models of SCLC

2018-03-06 | GSE110853 | GEO

Gene expression signature of the three major epidermal differentiation compartments: interfollicular epidermis (IFE), hair follicle (HF) and sebaceous gland (SG).

Project description:There are multiple stem cells in adult mammalian epidermis, but the mechanisms controlling lineage specification are poorly understood. To identify gene expression signatures of the three major epidermal differentiation compartments we micro-dissected individual SG, IFE and HF from adult epidermis. The RNA was isolated from age and sex matched wild-type mice and performed transcriptome analysis with Affymetrix Exon microarrays We micro-dissected individual interfollicular epidermis (IFE), hair follicle (HF) and sebaceous gland (SG) from adult tail epidermis. The RNA was isolated from age and sex matched wild-type mice. Three biological replicates for each epidermal differentiation compartment were analyzed.

2017-05-16 | E-GEOD-62604 | biostudies-arrayexpress

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