Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profiling of Drosophila melanogaster heads from experimentally-evolved populations

ABSTRACT: We used RNA sequencing to examine the transcriptomes of male and female heads from experimentally-evolved D. melanogaster populations after 117 generations of mating system manipulation in order to examine the pattern of evolution in sex-biased genes.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE50915 | GEO | 2014/03/17

SECONDARY ACCESSION(S): PRJNA219362

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptional profiling of Drosophila melanogaster heads from experimentally-evolved populations

Project description:We used RNA sequencing to examine the transcriptomes of male and female heads from experimentally-evolved D. melanogaster populations after 117 generations of mating system manipulation in order to examine the pattern of evolution in sex-biased genes. Examined head transcriptomes of 3 monogamous populations and 3 polygamous populations, both males and females, for 12 total samples.

2014-03-17 | E-GEOD-50915 | biostudies-arrayexpress

Gene expression divergence in experimentally evolved Drosophila pseudoobscura females

Project description:We used experimentally evolved Drosophila pseudoobscura females, who have experienced either enforced monogamy or increased polyandry for 100 generations, to assess divergence in their transcriptomic response to both mating system variation and mating experience.

2015-01-01 | GSE35410 | GEO

Experimentally-evolved male effects on female gene expression in the head and abdomen

Project description:We measured gene expression of D. melanogaster female heads and abdomens after mating with males from six populations evolved under either enforced monogamy (no male-male competition, 3 populations) or sustained polygamy (intense male-male competition, 3 populations).

2019-03-28 | GSE128403 | GEO

Post-mating expression of female Drosophila melanogaster genomes experimentally evolved under different post-copulatory sexual selection regimes

Project description:By combining an experimental evolution approach with genomic techniques, we investigated the effects of seminal fluid on female gene expression. In our study, we experimentally manipulated the mating system in replicate populations of D. melanogaster, by removing post-copulatory sexual selection, with the aim of testing differences in short term post-mating reaction of females evolved under different mating strategies. We show that monogamous females suffer decreased fecundity, regardless of the type of male they were mated with, and that their post-mating gene expression profiles differ significantly from promiscuous females, involving 1141 transcripts (9% of the genes tested). These transcripts are active in several tissues, mainly ovaries, neural tissues, midgut and spermathecae, and are involved in metabolic processes, reproduction and signaling pathways. Our results provide a list of candidate genes responsible for the decrease in female fecundity in the absence of post-copulatory sexual selection, and demonstrate how the female post-mating response can evolve under different mating systems over relatively short time frames. From an LHM base population, we created 8 replicate populations and maintained them under experimental evolution: 4 populations were allowed to mate only once every generation (monogamy), and the other 4 were kept under the standard mating protocol (promiscuous). After 46 generations, we crossed males and females within the same population and with individuals of the opposite treatment. Mated female flies were frozen 6 h after mating and RNA extracted. Two biological replicates per cross per population (2x2x8=32 samples).

2014-01-01 | E-GEOD-48385 | biostudies-arrayexpress

Post-mating expression of female Drosophila melanogaster genomes experimentally evolved under different post-copulatory sexual selection regimes

2014-01-01 | GSE48385 | GEO

Post-mating expression of female D. melanogaster genomes experimentally evolved under different post-copulatory sexual selection regimes

2014-01-15 | GSE30089 | GEO

Sex-biased expression in heads of three Drosophila species

Project description:Investigation of sex-biased expression across species have relied on measurements from whole flies which sample the extensive expression differences found in the germline and gonads of females and males. We wanted to examine genes with sex-biased expression in a somatic tissue to analyze patterns of genes with sex-biased expression in the context of a tissue more phenotypically similar between females and males. We used a Nimblegen microarray designed for Drosophila melanogaster and two custom micoarrays designed for D. pseudoobscura and D. mojavensis to survey gene expression differences in heads of females and males. PolyA+ mRNA was isolated in quadruplicate from dissected heads of 8 day old adult females and males. Female and male mRNA were labeled with Cy3 or Cy5 dyes separately and co-hybridized to species-specific microarrays. We analyzed a total of 24 channels of data.

2010-07-30 | E-GEOD-23309 | biostudies-arrayexpress

Sex-biased expression in heads of three Drosophila species

2010-07-30 | GSE23309 | GEO

Post-mating expression of female D. melanogaster genomes experimentally evolved under different post-copulatory sexual selection regimes

Project description:By combining an experimental evolution approach with genomic techniques, we investigated the effects of seminal fluid on female gene expression. In our study, we experimentally manipulated the mating system in replicate populations of D. melanogaster, by removing post-copulatory sexual selection, with the aim of testing differences in short term post-mating reaction of females evolved under different mating strategies. We show that monogamous females suffer decreased fecundity, regardless of the type of male they were mated with, and that their post-mating gene expression profiles differ significantly from promiscuous females, involving 1141 transcripts (9% of the genes tested). These transcripts are active in several tissues, mainly ovaries, neural tissues, midgut and spermathecae, and are involved in metabolic processes, reproduction and signaling pathways. Our results provide a list of candidate genes responsible for the decrease in female fecundity in the absence of post-copulatory sexual selection, and demonstrate how the female post-mating response can evolve under different mating systems over relatively short time frames. From a LHM base population, we created 8 replicate populations and maintained them under experimental evolution: 4 populations were allowed to mate only once every generation (monogamy), and the other 4 were kept under the standard mating protocol (promiscuous). After 46 generation, we crossed males and females within the same population and with individuals of the opposite treatment. Mated female flies were frozen 6 h after mating and RNA extracted. Two biological replicates per cross per population (2x2x8=32 samples).

2014-01-15 | E-GEOD-30089 | biostudies-arrayexpress

heads only

Project description:heads only

| PRJNA87985 | ENA