Advancing Functional Utility of PAR-CLIP by Quantifying Background Binding to mRNAs and lncRNAs

ABSTRACT: We developed a method for measuring non-specific background in PAR-CLIP data demonstrating that covalently crosslinked background binding is common, reproducible and apparently universal. Furthermore, we show that quantitative determination of background is essential for identifying targets of weakly binding RNA-binding proteins and can substantially improve motif analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE50989 | GEO | 2013/11/07

SECONDARY ACCESSION(S): PRJNA219483

REPOSITORIES: GEO