Transcriptomics,Genomics

Dataset Information

48

Expression data from WT and Eed KO neonatal mouse LT-HSC


ABSTRACT: Recent studies point to a pivotal role of polycomb repressive complex 2 (PRC2) in stem cell function and cancer. Loss of function approaches targeting individual PRC2 subunits have however generated findings that are difficult to reconcile. Here, we prevent assembly of both Ezh1- and Ezh2-containing PRC2 complexes by conditional deletion of Eed, a core subunit, and assess glodbal gene expression changs in LT-HSCs. Overall design: LT-HSC (long-term hematopoietic stem cells) were sorted from bone marrow of 9-day old wild type (Lin-Sca1+Kit+CD48-CD150+) or Eed KO animals (Lin-Sca1+CD48-CD150+). Each sample was double or triple sorted to ensure high purify.

INSTRUMENT(S): [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array

SUBMITTER: Huafeng Xie  

PROVIDER: GSE51084 | GEO | 2013-09-24

SECONDARY ACCESSION(S): PRJNA221146

REPOSITORIES: GEO

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Publications

Polycomb repressive complex 2 regulates normal hematopoietic stem cell function in a developmental-stage-specific manner.

Xie Huafeng H   Xu Jian J   Hsu Jessie H JH   Nguyen Minh M   Fujiwara Yuko Y   Peng Cong C   Orkin Stuart H SH  

Cell stem cell 20131114 1


Recent studies point to a pivotal role of Polycomb repressive complex 2 (PRC2) in stem cell function and cancer. Loss-of-function approaches targeting individual PRC2 subunits have, however, generated findings that are difficult to reconcile. Here, we prevent assembly of both Ezh1- and Ezh2-containing PRC2 complexes by conditional deletion of Eed, a core subunit, and assess hematopoiesis. We find that deletion of Eed exhausts adult bone marrow hematopoietic stem cells (HSCs), although fetal live  ...[more]

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