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Identification of CNA associated with bone metastatic breast cancer cells

ABSTRACT: We used comparative genomic hybridization (CGH) analysis between Bone metastatic derivatives isolated in vivo and parental cells and focused on genomic changes affecting the expression of potential bone metastasis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE51237 | GEO | 2014/10/01

SECONDARY ACCESSION(S): PRJNA221790

REPOSITORIES: GEO

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Identification of CNA associated with bone metastatic breast cancer cells

Project description:We used comparative genomic hybridization (CGH) analysis between Bone metastatic derivatives isolated in vivo and parental cells and focused on genomic changes affecting the expression of potential bone metastasis. Total RNA from biological and technical replicates of parental MCF7 and BoM2 bone metastasis derivatives grown for 48 hours in regular media (see growth protocol). High-molecular DNA was isolated from in vitro cultured MCF7 and BoM2 cells using GeneEluteM-bM-^DM-" Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich) following manufactureM-bM-^@M-^Ys instructions.

2014-10-01 | E-GEOD-51237 | biostudies-arrayexpress

Identification of genes associated with bone metastatic breast cancer cells

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Identification of genes associated with bone metastatic breast cancer cells

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Bone metastatic variant of MCF7 cells

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Project description:We herein examine the transciptioal alteration of a single cell derived MDA-MB-231 subline,SCP21. By retrieving tumor cells inoculated in different sites, we generated multiple mammary fat pad tumor, lung metastases or bone metastasis derived SCP21 sublines. RNA-sequencing uncovered the decreased expression of EZH2 target genes in bone metastases derived cells compared other organ entrained and parental SCP21 cells , suggesting enhanced EZH2 activity. Moreover, such phenotype is reversible upon in vitro culture. As a positive control, bone derived cells were also treated with EZH2 inhibitor, EPZ011989, to confirm the decreased level of EZH2 target genes upon EZH2 inhibition.

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Expression data of extracellular vesicles from bone metastatic breast cancer cell line

Project description:Bone metastasis is an incurable complication of breast cancer. In advanced stages, patients with estrogen-positive tumors experience significantly higher incidence of bone metastasis compared to estrogen-negative patients. To investigate how estrogen-positive breast cancer cells communicate with bone microenvironmental cells, extracellular vesicles from bone metastatic MCF7BoM2 were purified and analyzed. We used GeneChip miRNA 4.1 Array to detail the miRNA expression in the extracellular vesicles from the bone metastatic MCF7BoM2 and its parental cell line MCF7, and identified the dysregulated miRNAs.

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Bone invigorates metastatic seeding

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Expression data from human squamous cell lung cancer line HARA and highly bone metastatic subline HARA-B4.

Project description:We investigated the molecular mechanisms for osteolytic bone metastasis by selecting human lung cancer cell line subpopulations with elevated metastatic activity and validating genes that are overexpressed in these cells. A bone-seeking squamous lung cancer cell line (HARA-B4) was established by sequentially injecting parental HARA cells into the left ventricle of male 5-week-old nude mice 4 times. The parental line HARA and highly bone-metastatic line HARA-B4 were analized. Total of two samples. No replicates.

2011-05-19 | E-GEOD-29367 | biostudies-arrayexpress

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