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MRNA microarray analysis on HepG2 cells exposed to amorphous Silica Nanoparticles (SiO2-NP)

ABSTRACT: In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to amorphous Silica nanoparticles on Human hepatoma (HepG2) cells. We performed whole genome DNA microarray experiments using HepG2 cells exposed to for 24h. We used whole genome microarrays to screen for global changed in HepG2 transcription profiles and with subsequent quantitative analysis conducted on selected genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE51276 | GEO | 2013/10/01

SECONDARY ACCESSION(S): PRJNA222285

REPOSITORIES: GEO

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mRNA microarray analysis on HepG2 cells exposed to amorphous Silica Nanoparticles (SiO2-NP)

Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to amorphous Silica nanoparticles on Human hepatoma (HepG2) cells. We performed whole genome DNA microarray experiments using HepG2 cells exposed to for 24h. We used whole genome microarrays to screen for global changed in HepG2 transcription profiles and with subsequent quantitative analysis conducted on selected genes. 24h SiO2-NP (100 mg/L) exposed HepG2 cells were used for total RNA extraction and hybridization on Affymetrix microarrays.

2013-10-01 | E-GEOD-51276 | biostudies-arrayexpress

Expression data from human lung epithelial cells after amorphous silica nanoparticle exposure for 40 passages

2016-06-01 | GSE82062 | GEO

Expression data from human lung epithelial cells after amorphous silica nanoparticle exposure for 40 passages

Project description:Amorphous silica nanoparticles induce malignant transformation and tumorigenesis of human lung epithelial cells. We used microarrays to detail the global programme of gene expression underlying the cellular malignant transformation induced by amorphous silica nanoparticles and identified distinct classes of up-regulated and down-regulated genes during this process. The human lung epithelial cells, Beas-2B were continuously exposed to 5 Î¼g/mL amorphous silica nanoparticles for 40 passages, and named as BeasSiNPs-P40 (shortly as P40-5 during the further microarray detection). Meanwhile, the passage-matched control Beas-2B cells, named as Beas-P40 (shortly as NC during the further microarray detection).

2016-06-01 | E-GEOD-82062 | biostudies-arrayexpress

Macrophage response to silica nanoparticles

Project description:Using a macrophage cell line, we demonstrate the ability of amorphous silica particles to stimulate inflammatory protein secretion and induce cytotoxicity. Whole genome microarray analysis of early gene expression changes induced by 10nm and 500nm particles showed that the magnitude of change for the majority of genes correlated more tightly with particle surface area than either particle mass or number. Gene expression changes that were size-specific were also identified, however the overall biological processes represented by all gene expression changes were nearly identical, irrespective of particle diameter. Our results suggest that on an equivalent nominal surface area basis, common biological modes of action are expected for nano- and supranano-sized silica particles. Keywords: Dose-response study

2008-12-11 | GSE13005 | GEO

Transcription profiling of mouse macrophage response to silica nanoparticles

2009-01-13 | E-GEOD-13005 | biostudies-arrayexpress

Effect of ultra-small silica nanoparticles (USSNs) on gene expression of peripheral blood mononuclear cells

Project description:Humans are exposed to high levels of amorphous silica particles daily, as they are used in food, cosmetic and pharmaceutical products, and recently in bioclinical applications. Here, peripheral blood mononuclear cells were exposed to USSNs (silicon) and their gene expression was assessed

2018-04-13 | GSE113088 | GEO

Amorphous silica nanoparticle exposures can lead to mitochondrial protein changes

Project description:Mitochondrial fractions from J774 mouse macrophage/monocyte cells were exposed to amorphous silica nanoforms and mitochondrial protein profiles were examined to gain information on relative toxicities of these nanomaterials on mitochondrial pathways and identify potency determinants, for risk characterization. The applicability of this approach for screening mitochondrial toxicity of nanomaterials was also verified.

2023-03-22 | PXD027593 | Pride

Responses of macrophages to repeated exposures to different forms of amorphous silica

Project description:Synthetic amorphous silica (SAS) is a nanomaterial used in a wide variety of applications, including the use as a food additive. Two types of SAS are commonly employed as a powder additive, precipitated silica and fumed silica. Numerous studies have investigated the effects of synthetic amorphous silica on mammalian cells. However, most of them have used an exposure scheme based on a single dose of SAS. In this study, we have used instead a repeated 10-days exposure scheme, closer to the occupational exposure encountered in daily life. As a biological model we have used the murine macrophage cell line J774A.1, as macrophages are very important innate immune cells in the response to particulate materials. In order to get a better appraisal of the macrophage responses to this repeated exposure to SAS, we have used proteomics as a wide-scale approach. Furthermore, some of the biological pathways detected as modulated by the exposure to SAS by the proteomic experiments have been validated through targeted experiments. Overall, proteomics showed that precipitated SAS induced a more important macrophage response than fumed SAS at equal dose. Nevertheless, validation experiments showed that most of the responses detected by proteomics are indeed adaptive, as the cellular homeostasis appeared to be maintained at the end of the exposure. For example, the intracellular glutathione levels or the mitochondrial transmembrane potential at the end of the 10 days exposure were similar for SAS-exposed cells and for unexposed cells. Nevertheless, important functions of macrophages such as phagocytosis, TNF and interleukin-6 secretion were up-modulated after exposure, as was the expression of important membrane proteins such as the scavenger receptor or the MAC-1 receptor. These results suggest that repeated exposure to low doses of SAS slightly modulates the immune functions of macrophages, which may alter the homeostasis of the immune system.

2022-06-09 | PXD030002 | Pride

Dose dependent toxicological response of lung cells exposed to silica nanoparticles

Project description:We have employed whole genome microarray expression to distinguish the effect of Fumed Silica Nanoparticles on human alveolar epithelial A549 lung cells. Cells were exposed in vitro, and datasets of differentially expressed genes were identified for NPs versus control samples.

2015-03-01 | GSE63806 | GEO

Biocompatibility assessment of functionalized magnetic mesoporous silica nanoparticles in human HepaRG cells

Project description:We have employed whole genome microarray expression to distinguish the effect of diversely functionalized magnetic silica nanoparticles on human HepaRG cells. Cells were exposed in vitro, and datasets of differentially expressed genes were identified for NPs versus control samples.

2017-09-23 | GSE98236 | GEO

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