Transcriptomics,Genomics

Dataset Information

43

Sub-chronic transcriptional response in adult male MutaTMMouse following oral exposure to benzo(ghi)perylene in lung, liver, and forestomach tissues [BghiP]


ABSTRACT: Polycyclic aromatic hydrocarbons (PAHs) are a class of hundreds of structurally similar chemicals ubiquitously present in our environment. They are created during the incomplete combustion of organic materials, such as oil, wood, tobacco, and charbroiled meat. As such, human exposure to mixtures of PAHs can occur through consumption of PAH-containing foods and water, inhalation of polluted air, or dermal contact. Several PAHs have been classified as carcinogenic to humans or probably carcinogenic to humans by the International Agency for Research on Cancer. benzo(ghi)perylene is one such compound. In the present study, we sought to determine the dose-dependent changes in gene expression upon oral exposure to benz(a)anthracene in the lung, liver, and forestomach tissues. Adult male MutaTMMouse were exposed to three doses of benzo(ghi)perylene or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure. Overall design: This experiment examined the pulmonary, hepatic, and forestomach transcriptional response of male mice exposed to benzo(ghi)perylene for 28 days at three different doses, including D1 (6.25 mg/kg-bw/day), D2 (12.5 mg/kg-bw/day), and D3 (25 mg/kg-bw/day) and vehicle control (D0). Each dose group was examined 72 hours following the final exposure. Each dose group had 4-5 biological replicates. There were a total 20, 20, and 20 samples (arrays) from the lung, liver, and forestomach, respectively, included in the final analysis using a two-colour reference design.

INSTRUMENT(S): Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version)

SUBMITTER: Sarah Labib 

PROVIDER: GSE51316 | GEO | 2015-10-01

SECONDARY ACCESSION(S): PRJNA222399

REPOSITORIES: GEO

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Publications

Comparative transcriptomic analyses to scrutinize the assumption that genotoxic PAHs exert effects via a common mode of action.

Labib S S   Williams A A   Guo C H CH   Leingartner K K   Arlt V M VM   Schmeiser H H HH   Yauk C L CL   White P A PA   Halappanavar S S  

Archives of toxicology 20150916 10


In this study, the accuracy of the assumption that genotoxic, carcinogenic polycyclic aromatic hydrocarbons (PAHs) act via similar mechanisms of action as benzo(a)pyrene (BaP), the reference PAH used in the human health risk assessment of PAH-containing complex mixtures, was investigated. Adult male Muta™Mouse were gavaged for 28 days with seven individual, genotoxic PAHs. Global gene expression profiles in forestomach, liver, and lung (target tissues of exposure) were determined at 3 days post-  ...[more]

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