Single Cell RNA-sequencing of Pancreatic Circulating Tumor Cells

ABSTRACT: Circulating Tumor Cells (CTCs) are shed from primary tumors into the bloodstream, mediating the hematogenous spread of cancer to distant organs. Using a pancreatic cancer mouse model, we applied a microfluidic device to isolate CTCs independently of tumor epitopes, subjecting these to single cell RNA-sequencing. This study was conducted to determine the heterogeneity of pancreatic CTCs and to compare these CTCs to matched primary tumors, cell line controls (NB508 cancer cell line and MEF non-cancer cell line), primary tumor single cells, and normal leukocytes/WBCs.

ORGANISM(S): Mus musculus

PROVIDER: GSE51372 | GEO | 2014/09/22

SECONDARY ACCESSION(S): PRJNA222842

REPOSITORIES: GEO