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PAO1 gene expression at 22°C and 37°C in Lysogeny broth

ABSTRACT: Pseudomonas aeruginosa strain PAO1 was grown at 22°C and 37°C in Lysogeny broth (LB) and RNA was hybridized on the Affymetrix P. aeruginosa chip.

ORGANISM(S): Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1

PROVIDER: GSE51409 | GEO | 2014/01/01

SECONDARY ACCESSION(S): PRJNA222984

REPOSITORIES: GEO

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PAO1 gene expression at 22M-BM-0C and 37M-BM-0C in Lysogeny broth

Project description:Pseudomonas aeruginosa strain PAO1 was grown at 22M-BM-0C and 37M-BM-0C in Lysogeny broth (LB) and RNA was hybridized on the Affymetrix P. aeruginosa chip. PAO1 was grown in triplicate in Lysogeny broth at 22M-BM-0C or 37M-BM-0C. Total RNA from each sample was pooled and amplified then assayed in triplicate resulting in 6 total samples.

2014-01-01 | E-GEOD-51409 | biostudies-arrayexpress

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Project description:In this experiment the transcriptional response of the opportunistic human pathogen Pseudomonas aeruginosa towards physiological concentrations of the major human host defense peptide LL-37 was investigated using microarrays. To this aim, three independent cultures of P. aeruginosa PAO1 were grown until mid-log phase in Mueller-Hinton broth and subsequently incubated with either sublethal LL-37 concencentrations (20 M-5g/ml) or without peptide for 2 h at 37 M-0C following RNA extraction and microarray analysis.

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Project description:Pseudomonas aeruginosa strains PAHM4 and PAO1 were grown at 37C on LB and RNA was hybridized on the Affymetrix P. aeruginosa chip to compare transcript differences from a BQ isolate to a well characterized wound isolate. Strains PAHM4 and PAO1 were grown in triplicate. Total RNA from each sample was pooled and amplified then assayed in triplicate resulting in 6 total samples. PAO1 is the control strain while PAHM4 is the experimental strain.

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P. aeruginosa PAO1 WT and PA2663 biofilm cells

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Pseudomonas aeruginosa PAO1 response to sphingomyelin

Project description:Analysis of Pseudomonas aeruginosa PAO1 treated with 200 µM sphingomyelin. Results provide insight into the response to sphingomyelin in P. aeruginosa.

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Temperature dependent gene expression levels at 28°C and 37°C for Pseudomonas aeruginosa PAO1

Project description:To further understand the gene expression characteristics of Pseudomonas aeruginosa PAO1, we have applied whole genome microarray expression profiling as a discovery platform to specify the temperature dependent expression of PAO1 genome at soil and human body temperature. We selected 28°C as temperature representative of the soil niche and 37°C for human body. The results from the temperature dependent transcriptome analysis are consistent to our previous published data that the phzM, ptsP and lasI genes expression is upregulated at 37°C [11]. The comparison analysis of the M18 genome expressional profiles at 28°C and 37°C indicated a total of 596 genes expressed in a temperature dependent manner over two fold. Cells were grown to OD600=5.0-6.0 (late exponential phase) in LB medium at 28℃ and 37℃, respectively. Three independent experiments were performed at each time.

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Expression data of Pseudomonas aeruginosa cells in planktonic or biofilm mode of growth

Project description:Biofilm formation by Pseudomonas aeruginosa relies on specific changes in gene expression. Some of these genes, for instance, control antibiotic resistance. We used microarrays to detail the global programme of gene expression underlying biofilm formation and identified distinct classes of up-regulated genes during this process. Pseudomonas aeruginosa PAO1 cells were grown as planktonic cells in LB broth for 4 hours (PC4) or 24 hours (PC24) and sessile cels for 24 hours.

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