Genomics

Dataset Information

2

ADAM9 Up-regulates N-Cadherin via MiR-218 Suppression in Lung Adenocarcinoma Cells


ABSTRACT: Identification of miRNAs that differentially expressed in brain metastatic cells and were predicted to target CDH2. To investigate which miRNAs could regulate CDH2 expression in brain-metastatic lung cancer cells, we examined the miRNA expression profiles in brain-metastatic lung cancer cells and their parental cells using Illumina miRNA microarray. By comparing the endogenous expression of all miRNAs, 146 miRNAs were selected by 2-fold changes in brain-metastatic lung cancer cells. Furthermore, we used several algorithms in miRSystem to predict which miRNA whose target gene was CDH2. There were 44 miRNAs that were predicted to target CDH2. In total, we identified nine miRNAs whose target gene was CDH2 and whose expression was significantly different between the highly metastatic cells and their parental cells (Fig. 2A). Among these miRNAs, four miRNAs were found to be down-regulated and five miRNAs were up-regulated in brain-metastatic lung cancer cells (Fig. 2B). Overall design: Total RNA obtained from human lung cancer cells (F4) compared to brain metastasis lung cancer cells (BM).

INSTRUMENT(S): Illumina Human v2 MicroRNA expression beadchip

ORGANISM(S): Homo Sapiens

SUBMITTER: Liang-Chuan Lai  

PROVIDER: GSE51666 | GEO | 2016-10-11

SECONDARY ACCESSION(S): PRJNA225790

REPOSITORIES: GEO

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Publications

ADAM9 up-regulates N-cadherin via miR-218 suppression in lung adenocarcinoma cells.

Sher Yuh-Pyng YP   Wang Li-Ju LJ   Chuang Li-Ling LL   Tsai Mong-Hsun MH   Kuo Ting-Ting TT   Huang Cheng-Chung CC   Chuang Eric Y EY   Lai Liang-Chuan LC  

PloS one 20140404 4


Lung cancer is the leading cause of cancer death worldwide, and brain metastasis is a major cause of morbidity and mortality in lung cancer. CDH2 (N-cadherin, a mesenchymal marker of the epithelial-mesenchymal transition) and ADAM9 (a type I transmembrane protein) are related to lung cancer brain metastasis; however, it is unclear how they interact to mediate this metastasis. Because microRNAs regulate many biological functions and disease processes (e.g., cancer) by down-regulating their target  ...[more]

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