Real-time quantitative PCR analysis of human colonic HT-29 cells under insulin stimulation

ABSTRACT: Compared mRNA expression between control and insulin treated cells. HT29 cells were treated with 100nM Insulin for 24h. The RT2 Profiler PCR Arrays PAHS-30C (SA Biosciences, MD, USA) was designed to analyze 84 genes related to human insulin signaling pathway. The RT-PCR was carried out using an ABI PRISM® 7000 Sequence Detection System (Applied Biosystems, Foster City, CA).The expression of HSD11B2 for the 3 experiments concerned was monitored in parallel by real time PCR which confirming significant downregulation by insulin.

ORGANISM(S): Homo sapiens

PROVIDER: GSE51677 | GEO | 2014/06/01

SECONDARY ACCESSION(S): PRJNA224313

REPOSITORIES: GEO