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Analysis of gene expression in sublines of DU145 human prostate cancer cells created through in vivo metastatic selection

ABSTRACT: DU145 human prostate cancer cells were injected into the prostate of nude mice and cells metastasizing to lymph node (LN1) grown and experiment repeated to make LN2-4. DU145 human prostate cancer cells were injected into the tail vein of nude mice and cells metastasizing to lung (ivLU1) grown and experiment repeated to make ivLU2-4.

ORGANISM(S): Homo sapiens

PROVIDER: GSE51755 | GEO | 2013/10/29

SECONDARY ACCESSION(S): PRJNA224959

REPOSITORIES: GEO

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Analysis of gene expression in sublines of DU145 human prostate cancer cells created through in vivo metastatic selection

Project description:DU145 human prostate cancer cells were injected into the prostate of nude mice and cells metastasizing to lymph node (LN1) grown and experiment repeated to make LN2-4. DU145 human prostate cancer cells were injected into the tail vein of nude mice and cells metastasizing to lung (ivLU1) grown and experiment repeated to make ivLU2-4. 8 groups representing individual cell lines

2013-10-29 | E-GEOD-51755 | biostudies-arrayexpress

MicroRNA expression in DU145 sublines metastatic to lymph nodes

Project description:Comparison of non-coding RNA profiling by array in sublines of DU145 human prostate cancer cell lines created by in vivo cycling Cell lines created by removal and growth of metastatic human DU145 tumor cells from mouse lymph node (metastasized from prostate xenograft) for LN cells and extracted from lung after intravenous injection (ivLU cells). Cell line numebr represented number of in vivo cycles of metastatic selection

2013-10-29 | GSE51753 | GEO

MicroRNA expression in DU145 sublines metastatic to lymph nodes

2013-10-29 | E-GEOD-51753 | biostudies-arrayexpress

RNA-seq of human prostate cancer cell lines grown as orthotopic tumour models of castration resistant prostate cancer

Project description:To identify changes at multiple omic levels between hormone naive and castration resistant prostate cancer. Using orthograft murine tumour models of human cell lines injected into the prostate of CD-1 Nude mice grown under castration resistant (castrate) or hormone naive (non-castrate) conditions.

2021-05-03 | E-MTAB-9831 | biostudies-arrayexpress

Comparative analysis of gene expression of cell line DU145 grown under sphere forming condition and as monolayer

Project description:By employing a global gene expression profiling, we performed analysis of the molecular pathways which are deregulated in prostate cancer cell line DU145 grown under tumorsphere forming condition and as standard attached monolayer culture. Both oriignal DU145 and its radioresistant derivative DU145-RR were analyzed

2021-07-01 | GSE148013 | GEO

Next generation RNA sequencing elucidates the antitumor effects of Zelnorm via immune microenvironment regulation using DU145 tumor model

Project description:Prostate cancer DU145 tumors were subcutaneously established in male nude mice. In nude mice, Zelnorm were orally administrated. Tumor volume and body weight were assessed (tumor volume=1/2 (length×width2)). Tumors were excised and weighted. Tumor samples were profiled by RNA-seq analysis. Respectively, to differentiate the gene expression originated from human (cancer cells) or mouse (tumor TME part), data were mapped against human (GRCh38) and mouse (GRCm38) genomes separately.

2023-12-31 | GSE198765 | GEO

Comparative analysis of gene expression of cell lines DU145 andLNCaP grown under standard and glutamine deprived condition

Project description:By employing a global gene expression profiling, we performed analysis of the molecular pathways which are deregulated in prostate cancer cell lines DU145 and LNCaP grown under standard and glutamine deprived condition. Both oriignal cell lines and their radioresistant derivatives DU145-RR and LNCaP-RR were analyzed

2021-07-01 | GSE148016 | GEO

The effect of GPR30 agonist G-1 on androgen-sensitive and castration-resistant LNCaP xenografts

Project description:G-1 is an agonist to GPR30. Activation of GPR30 by G-1 inhibited prostate cancer cell growth in LNCaP xenografts regrown after catration of the host (nude mice), but not in the androgen-sensitive LNCaP xenograft grown in an intact host. Results provide insights into the molecular basis of G-1 action in castration-resistant prostate cancer. Male nude mice were injected with LNCaP cells. When the LNCaP tumors reached 150–300 mm3, mice were divided into two groups: intact (androgen-sensitive tumor) and castrated. For the intact group, mice were subcutaneously injected with vehicle alone (95% PBS, 2.5% DMSO, 2.5% ethanol) or G-1 (4 mg/kg/day in vehicle) daily for 16 days. For the castrated group, tumors regressed and then regrew to ~300-400mm3. Mice were treated daily with vehicle or G-1 as described for 16 days. Tumors were harvested for RNA extraction and microarray experiments.

2014-02-14 | E-GEOD-54974 | biostudies-arrayexpress

22Rv1 in vivo progression model

Project description:Purpose: Even in last stage of metastatic castration-resistant prostate cancer, androgen receptor (AR) signaling remains active.To derive high metastatic prostate cancer (PCa), we labeled AR-positive but castration-resistant 22Rv1 PCa cells with luciferase gene (22Rv1-Luc2) and these cells were orthotopically implanted in mouse prostate for spontaneous progression. Methods: 2 × 10^5 of luciferase-expressing 22Rv1 cells (22Rv1-Luc2) cells were implanted in the anterior prostate of nude mice. After 12-14 weeks, the host mice were necropsied and the metastases from lumbar lymph nodes and primary tumors were dissected under laminar flow. Tumor tissues were minced using sterile scalpels and further digested with Collagenase D for 1 h. The lymph node metastatic cancer cells, named 22Rv1-M1, were orthotopically reimplanted in nude mice. At 12 weeks, the secondary metastases were isolated in the lumbar lymph nodes and designated as 22Rv1-M2 cells. Suspension of 1 × 10^6 22Rv1-M2 cells in DPBS was injected into nude mice through the tail vein, and mice developed metastases (22Rv1-M3) after 6 week. This procedure was repeated once to attain the 22Rv1-M4. Results: 22Rv1-derived metastatic cell lines exhibit increased in vitro and in vivo invasion activity as the progression from 22Rv1 to M4. Transcriptomic analysis of genome-wide gene expression in the M4 tumors reveal the unique gene expression profile compared to 22Rv1 tumors. Conclusions: Transcriptomic data provide the gene network for decoding the mechanism of PCa metastasis.

2017-12-01 | GSE99857 | GEO

Epigenome analysis of DU145 samples [II]

Project description:The Illumina Infinium 850k Human DNA methylation BeadChip was used to obtain DNA methylation profiles in DU145 cell samples. Samples included normal, PLK1 knockdown and UHRF1 knockdown DU145 cell samples. Each sample is repeated three times.

2022-10-31 | GSE185400 | GEO

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