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Re-establishment of desiccation tolerance in protruded radicles of Medicago truncatula Wt and abi5 seeds

ABSTRACT: Desiccation tolerance (DT) is the capacity to withstand total loss of cellular water. This property is acquired during seed filling and lost just after germination. However, in many species, a germinated seed can regain DT under adverse conditions such as osmotic stress. We discovered that the germinated seeds of the abscisic acid insensitive 5 (Mtabi5) mutant of Medicago truncatula lost their ability to re-establish DT during osmotic stress. To characterize the molecular processes that are influenced by MtABI5 during the re-establishment of DT tolerance, a transcriptome analysis was performed on the protruded radicles of germinated Mtabi5 mutants and wilt type before and after an osmotic treatment.

ORGANISM(S): Medicago truncatula

PROVIDER: GSE51830 | GEO | 2013/10/30

SECONDARY ACCESSION(S): PRJNA225525

REPOSITORIES: GEO

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Re-establishment of desiccation tolerance in protruded radicles of Medicago truncatula Wt and abi5 seeds

Project description:Desiccation tolerance (DT) is the capacity to withstand total loss of cellular water. This property is acquired during seed filling and lost just after germination. However, in many species, a germinated seed can regain DT under adverse conditions such as osmotic stress. We discovered that the germinated seeds of the abscisic acid insensitive 5 (Mtabi5) mutant of Medicago truncatula lost their ability to re-establish DT during osmotic stress. To characterize the molecular processes that are influenced by MtABI5 during the re-establishment of DT tolerance, a transcriptome analysis was performed on the protruded radicles of germinated Mtabi5 mutants and wilt type before and after an osmotic treatment. Six-condition experiment, R108_T versus R108_P, abi5-1_T versus abi5-1_P, abi5-2_T versus abi5-2_P. Biological replicates: 9 controls (R108-T, abi5-1_T, abi5-2_T), 9 treatments (R108-P, abi5-1_P, abi5-2_P), independently grown and harvested. One replicate per array.

2013-10-30 | E-GEOD-51830 | biostudies-arrayexpress

Genome-wide analysis of desiccation tolerance-related genes in Arabidopsis seeds

Project description:The combination of robust physiological models with “omics” studies holds promise for the discovery of genes and pathways linked to how organisms deal with drying. Here we used a transcriptomics approach in combination with an in vivo physiological model of re-establishment of desiccation tolerance (DT) in Arabidopsis thaliana seeds. We show that the incubation of desiccation-sensitive (DS) germinated Arabidopsis seeds in a polyethylene glycol (PEG) solution re-induces the mechanisms necessary for expression of DT. Based on a SNP-tile array gene expression profile, our data indicates that the re-establishment of DT, in this system, is related to a programmed reversion from a metabolic active to a quiescent state similar to prior to germination. Our findings show that transcripts of germinated seeds after the PEG treatment are dominated by those encoding LEA, seed storage and dormancy-related proteins. On the other hand, a massive repression of genes belonging to many other classes such as photosynthesis, cell wall modification and energy metabolism occurs in parallel. Furthermore, comparison with a similar system for Medicago truncatula reveals a significant overlap between the two transcriptomes. Such overlap may highlight core mechanisms and key regulators of the trait DT. Taking into account the availability of the many genetic and molecular resources for Arabidopsis, the described system may prove useful for unraveling DT in higher plants.

2013-06-21 | GSE30853 | GEO

Medicago truncatula

Project description:Re-establishment of desiccation tolerance in protruded radicles of Medicago truncatula Wt and abi5 seeds

| PRJNA225525 | ENA

Genome-wide analysis of desiccation tolerance-related genes in Arabidopsis seeds

2013-06-21 | E-GEOD-30853 | biostudies-arrayexpress

Transcription profiling of Medicago truncatula radicles incubated in polyethylene glycol solution and embryos at different time points after pollination to study dessication tolerance

Project description:Time course of the transcriptome of desiccation-sensitive 2.7-2.9 mm-long radicles of Medicago truncatula seeds at different time points during incubation in a polyethylene glycol (PEG) solution at -1.7 MPa and 10°C, resulting in a gradual re-establishment of desiccation tolerance. Gene profiling was also performed on embryos before (14 days after pollination) and after acquisition of desiccation tolerance during maturation (20 days after pollination).

2006-04-30 | E-MEXP-664 | biostudies-arrayexpress

Desiccation Tolerance of Arabidopsis thaliana seeds

Project description:Desiccation tolerance (DT) allowed seed plants to conquer ecosystems with long periods of limited water availability. This adaptive features allows seeds to remain dried for very long times without losing their ability to germinate. There is little information about all the signaling components required to achieve DT and on how transcription factors (TFs) modulate global DT processes. We performed RNA-seq experiment and carbohydrates profiles of lec1, lec2, fus3 and abi3, as well as their corresponding wild types, at three stages of seed development 15, 17 and 21 DAF (day after open flower) belonging to the seed desiccation period. A complex experimental design approach and regulatory networks prediction were used to identify differentially expressed genes specifically involved in DT process.

2016-09-06 | GSE76015 | GEO

Integrative analysis of transcriptome, microRNA-seq and metabolome reveals an essential role of exogenous melatonin in enhancing salt stress tolerance during seed germination in maize [miRNA-seq]

Project description:To investigate the role of melatonin in salt tolerance of maize, we determine the seed germinated rate of maize under CK, NaCl and NaCl+melatonin. We then performed miRNA profiling analysis using data obtained from miRNA-seq of the seeds of three different treatments.

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Integrative analysis of transcriptome, microRNA-seq and metabolome reveals an essential role of exogenous melatonin in enhancing salt stress tolerance during seed germination in maize [RNA-seq]

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2023-05-10 | GSE223821 | GEO

Re-establishment of desiccation tolerance by PEG in germinated Arabidopsis thaliana seeds

Project description:Mature seeds of Arabidopsis thaliana are desiccation tolerant, but they lose DT while progressing to germination. Yet, there is a small developmental window during which DT can be rescued by treatment with polyethylene glycol (PEG). We used microarrays to identify relevant genes in the re-establishment of desiccation tolerance by PEG.

2015-08-25 | GSE64227 | GEO

RNAseq on 1mm and 5 mm germinating seeds with/without PEG treatment from dissected radicles and cotyledons

Project description:RNAseq from Medicago truncatula early post germination (1 and 5mm radicle seeds) from dissected radicles and cotyledons with and without PEG treatment to identify genes induced during the reinduction of the desiccation tolerance at early germination. Dessication tolerant tissues were radicles from 1mm radicle seeds treated with PEG: R1P, and cotyledons from 1- and 5-mm radicle seeds treated or not with PEG: C1, C5, C1P, C5P and desiccation sensitive tissues were radicles from 1mm radicle seeds (R1) and 5mm radicle seeds (R5).

2022-10-03 | GSE214468 | GEO

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