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Effects of exercise on gene expression level in human monocytes

ABSTRACT: We tested the hypothesis on the mechanisms responsible for the early control of monocytes function by identifying a discrete set of genes in circulating monocytes that were altered by exercise. Exercise leads to a rapid change in the profile of gene expression in monocytes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE51835 | GEO | 2014/02/15

SECONDARY ACCESSION(S): PRJNA225579

REPOSITORIES: GEO

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Effects of exercise on gene expression level in human monocytes

Project description:We tested the hypothesis on the mechanisms responsible for the early control of monocytes function by identifying a discrete set of genes in circulating monocytes that were altered by exercise. Exercise leads to a rapid change in the profile of gene expression in monocytes. Twelve healthy men (22-30 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 82% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. Monocytes were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Monocyte Isolation Kit II human #130-091-153 and the autoMACS√Ç¬Æ Pro Separator). Total RNA was extracted using TRIzol. For this study we used Affymetrix plus 2 (total of 24 chips).

2014-02-15 | E-GEOD-51835 | biostudies-arrayexpress

Effects of exercise on gene expression level in human NK Cells

2013-04-02 | GSE41914 | GEO

Effects of exercise on microRNA expression level in human monocytes

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Effects of exercise on microRNA expression level in human monocytes

Project description:Exercise leads to a rapid change in the profile of gene expression in monocytes. We hypothesized that microRNA expression in circulating monocytes would also be affected by brief exercise. Twelve healthy men (22-30yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about82% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. Monocytes were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Monocyte Isolation Kit II, human #130-091-153 and the autoMACSM-BM-. Pro Separator). Total RNA was extracted using TRIzolM-BM-.. For this study we used Agilent Human miRNA microarrays V2 (total of 24 arrays).

2014-02-15 | E-GEOD-51836 | biostudies-arrayexpress

Effects of exercise on gene expression level in human NK Cells

Project description:We tested the hypothesis on the mechanisms responsible for the early control of NK cell function by identifying a discrete set of genes in circulating NK cells that were altered by exercise. Exercise leads to a rapid change in the profile of gene expression in NK cells. Twelve healthy men (20-29 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 77% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACSM-BM-. Pro Separator). Total RNA was extracted using TRIzolM-BM-.. For this study we used Affymetrix plus 2 (total of 24 chips).

2013-04-02 | E-GEOD-41914 | biostudies-arrayexpress

Effects of exercise on miRNA expression level in human neutrophils

2010-03-31 | GSE18999 | GEO

Effects of exercise on miRNA expression level in human neutrophils

Project description:Exercise leads to a rapid change in the profile of gene expression in circulating neutrophils. We hypothesized that miRNA expression in circulating neutrophils would also be affected by brief exercise. Eleven healthy men (19-30 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 76% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. Neutrophils were isolated using OptiPrep Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzolM-BM-.. For this study we used Agilent Human miRNA microarrays V2 (total of 22 chips).

2010-03-31 | E-GEOD-18999 | biostudies-arrayexpress

Effects of exercise on miRNA expression level in human NK Cells

2013-04-02 | GSE41913 | GEO

Effects of exercise on miRNA expression level in human NK Cells

Project description:Exercise leads to a rapid change in the profile of gene expression in NK cells. We hypothesized that microRNA expression in circulating NK cells would also be affected by brief exercise. Eleven healthy men (20-29 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 77% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACSM-BM-. Pro Separator). Total RNA was extracted using TRIzolM-BM-.. For this study we used Agilent Human miRNA microarrays V2 (total of 22 chips).

2013-04-02 | E-GEOD-41913 | biostudies-arrayexpress

Effects of exercise on miRNA expression in human peripheral blood mononuclear cells (PBMCs)

Project description:Exercise leads to a rapid change in the profile of gene expression in circulating PBMCs. We hypothesized that miRNA expression in circulating PBMCs would also be affected by brief exercise.

2011-09-26 | GSE28745 | GEO

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