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Sex-, stage-, and tissue-specific alternative splicing in Drosophila melanogaster

ABSTRACT: We used long-oligonucleotide microarrays to investigate whether alternative splicing in Drosophila is regulated in a sex-, stage-, or tissue-specific manner. To examine sex-specific splicing, we compared gene expression profiles of male and female pupae 12 hours after pupariation. To examine stage-specific splicing, we compared expression profiles of mixed-sex, 0-24 hour old embryos and mixed-sex, 12 hour old pupae. To examine tissue-specific splicing, we compared expression profiles of adult male heads and abdomens 24-48 hours after eclosion. To examine tissue-specific splicing, we compared expression profiles of adult male heads and abdomens at 24-48 hours after eclosion. Keywords: tissue-specific expression profiles

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE5190 | GEO | 2007/06/22

SECONDARY ACCESSION(S): PRJNA96529

REPOSITORIES: GEO

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Sex-, stage-, and tissue-specific alternative splicing in Drosophila melanogaster

Project description:We used long-oligonucleotide microarrays to investigate whether alternative splicing in Drosophila is regulated in a sex-, stage-, or tissue-specific manner. To examine sex-specific splicing, we compared gene expression profiles of male and female pupae 12 hours after pupariation. To examine stage-specific splicing, we compared expression profiles of mixed-sex, 0-24 hour old embryos and mixed-sex, 12 hour old pupae. To examine tissue-specific splicing, we compared expression profiles of adult male heads and abdomens 24-48 hours after eclosion. To examine tissue-specific splicing, we compared expression profiles of adult male heads and abdomens at 24-48 hours after eclosion. Keywords: tissue-specific expression profiles Drosophila isogenic line WI89 was used. Mixed-sex, mixed-stage embryos were harvested from plates on which females had been allowed to oviposit for 24 hours. To obtain synchronized cohorts of pupae, male and female white prepupae were collected at 0-1 hour after pupariation and aged for 12 hours at 25C. Mixed-sex pupal samples were generated by mixing equal amount of male and female pupal RNA. Adult heads and abdomens were dissected from 24-48 hour old males. mRNA was isolated and labeled without amplification.

2010-06-10 | E-GEOD-5190 | biostudies-arrayexpress

mRNA-Seq of brain tissue from honeybee (Apis mellifera) drones (male) and workers (female)

Project description:Transcriptional profiling of brain tissue of was performed by RNA-SEQ in females and males. The sex specific expressed and spliced genes were examined comparing drone and worker honeybees. Examination of mRNA profiles in heads of male and female honeybees pupae were compared.

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Aedes aegypti

Project description:Sex-specific gene expression profiles of Aedes aegypti 24 hr pupal heads

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Effects of ploidy and sex-locus genotype on gene expression patterns in the fire ant Solenopsis invicta [11d]

Project description:In Hymenoptera, sex is determined by a single-locus complementary sex determining system (sl-CSD). Males are normally haploid (hemizygous at the sl-CSD locus) but if the genotype of the sl-CSD locus is homozygous, they develop into diploid males. Here, we study the effects of ploidy and the sl-CSD-locus genotype by comparing gene expression differences between haploid males, diploid males and virgin queens at three developmental stages, pupae, 1 day and 11 days after eclosion. Keywords: diploid males, sl-CSD, ploidy-spcific gene, sex-specific gene, doublesex, sex-biased gene

2012-12-06 | GSE42785 | GEO

Effects of ploidy and sex-locus genotype on gene expression patterns in the fire ant Solenopsis invicta [1d]

2012-12-06 | GSE42779 | GEO

Effects of ploidy and sex-locus genotype on gene expression patterns in the fire ant Solenopsis invicta [pupa]

2012-12-06 | GSE42494 | GEO

Identification of genes contributing to a long circadian period in Drosophila melanogaster

Project description:Our objective was to identify candidate genes that contribute to the long 31-hour circadian period previously observed in DGRP_892. We performed transcriptional profiling of whole fly heads from two genotypes: DGRP_892, and Canton-S B, a line with a normal 24-hour circadian period. We collected fly heads every two hours over a 24-hour period. We quantified differential expression among genotype, time, and sex.

2020-12-04 | GSE155018 | GEO

Sex-specific gene expression profiles of Aedes aegypti 24 hr pupal heads

Project description:Custom microarrays were used to examine global differences in female vs. male gene expression in the developing pupal head of the dengue vector mosquito Aedes aegypti. RNA was extracted from the heads of male and female 24 hr pupae. 20 male or female heads were pooled for each of four replicates. Hybridization experiments were performed on the Nimblegen Aedes aegypti 12-plex microarray design: 090305_Aedes_aegypti_TEfam_expr.ndf. Four unique replicates and two repeat replicates were assessed in the hybridization experiment.

2014-10-27 | E-GEOD-56521 | biostudies-arrayexpress

Sex-specific mRNA and miRNA expression data from Drosophila larvae, pupae and adults [miRNA]

Project description:Sex differences in gene expression throughout development are poorly understood, especially sex-specific expression of micro RNAs. However these patterns of gene expression could have important implications in our understanding of the underlying mechanics of sex differentiation and sexual conflict. We extract mRNA and miRNA from male and female Drosophila melanogaster from three developmental timepoints, third larval instar, pupae and adults, and examine gene expression using microarrays. We found a large number of sex-biased mRNA transcripts at each stage of development, whereas sex-biased miRNA expression was low in larvae and pupae and more prevalent in adults.

2014-05-07 | GSE57321 | GEO

Sex-specific mRNA and miRNA expression data from Drosophila larvae, pupae and adults [mRNA]

2014-05-07 | GSE57320 | GEO

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