Systematic evaluation of DNase-seq experimental parameters

ABSTRACT: We systematicaly evaluated two major factors, enzyme digestion level and fragment size, that would affect DNase-seq experiment. We found that while under- or over-digestion sigificantly decreases the DNase-seq signal, there is a broad range of suitable digestion level. In addition, we found fragment smaller than nucleosome size is optimal to identify transcription factor binding events.

ORGANISM(S): Homo sapiens

PROVIDER: GSE51915 | GEO | 2013/11/25

SECONDARY ACCESSION(S): PRJNA225824

REPOSITORIES: GEO