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Expression data from cell lines treated with enavatuzumab and human IgG1 control

ABSTRACT: Cancer lines were treated with enavatuzumab and a human IgG1 control (MSL109) in vitro. Samples were extracted at various time points and analyzed via genechip. We used microarrays to examine gene expression patterns following treatment with enavatuzamab. Expression changes over time were compared with corresponding data from samples treated with a control.

ORGANISM(S): Homo sapiens

PROVIDER: GSE51934 | GEO | 2013/10/31

SECONDARY ACCESSION(S): PRJNA225836

REPOSITORIES: GEO

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Expression data from cell lines treated with enavatuzumab and human IgG1 control

Project description:Cancer lines were treated with enavatuzumab and a human IgG1 control (MSL109) in vitro. Samples were extracted at various time points and analyzed via genechip. We used microarrays to examine gene expression patterns following treatment with enavatuzamab. Expression changes over time were compared with corresponding data from samples treated with a control. 16 samples were analyzed for each cell line, with duplicate samples at 4 different time points (from 6 hours to 3 days) for each of the 2 treatments (enavatuzumab and control).

2013-10-31 | E-GEOD-51934 | biostudies-arrayexpress

Transcriptome analysis of mouse IgG1 memory B cell subsets

Project description:IgE plays an essential role in the pathogenesis of allergies and its production is strongly regulated. A transient IgE germinal center phase and lack of IgE memory cells limit the generation of pathogenic IgE, but this can be overcome by sequential switching of IgG1 cells to IgE. We investigated which population of IgG1 cells can give rise to IgE-producing cells in memory responses. We identified three populations of IgG1 memory B cells (DP:CD73+CD80+, SP:CD73-CD80+, DN:CD73-CD80-) that generate IgE plasma cells of high or low affinity, but none gives rise to IgE germinal center cells or IgE memory cells. The two memory IgG1 populations differ however in their ability to differentiate into IgG1 plasma cells and germinal center cells, and to expand the IgG1 memory B cell pool. To explore the molecular mechanisms that may explain the distinct functions of IgG1 memory B cell subsets we compared their expression by transcriptome analysis using next generation sequencing.

2017-07-03 | GSE83436 | GEO

Expression analysis of white adipose, brown adipose tissue and gastrocnemius muscle isolated from tumor bearing mice treated with control IgG1 or anti-GFRAL 3P10

Project description:RNA sequencing of white adipose, brown adipose tissue and gastrocnemius muscle isolated from non-tumor bearing and HT1080 tumor-bearing mice treated with control IgG1 or anti-GFRAL 3P10

2020-07-20 | GSE149263 | GEO

Expression data from IgE+ and IgG1+ B lymphocytes in mice infected with Nippostrongylus brasiliensis

Project description:The mechanisms involved in the maintenance of memory IgE responses are poorly understood, and the role played by germinal center (GC) IgE cells in these memory responses is particularly unclear. IgE B-cell differentiation is characterized by a transient GC phase, a bias towards the plasma cell (PC) fate, and dependence on sequential switching for the production of high-affinity IgE. We show here that IgE GC B cells are unfit to undergo the conventional GC differentiation program due to impaired B-cell receptor function and increased apoptosis. IgE GC cells fail to populate the GC light zone and are unable to contribute to the memory and long-lived PC compartments. Furthermore, we demonstrate that direct and sequential switching are linked to distinct B-cell differentiation fates: direct switching generates IgE GC cells, whereas sequential switching gives rise to IgE plasma cells. We propose a comprehensive model for the generation and memory of IgE responses. The purpose of this analysis was to: 1) identify expression differences between IgE and IgG1 B lymphocytes, 2) identify GC Dark Zone (DZ) and Light Zone (LZ) signatures of IgG1 GC cells. For that purpose, we compared in one experiment the gene expression patterns of IgE germinal center (GC) cells, IgG1 GC cells, IgE plasma cells (PC), IgG1 PC and naïve cells. In a second experiment, we compared the expression of IgG1 DZ GC cells with that of IgG1 LZ GC cells. Triplicates obtained from independent sorting experiments were used for all samples except two (IgG1 PC=2 samples; IgE PC=4 samples). Each sample was obtained from a pool of three individual mice. The mice used in the experiment were CeGFP BALB/c mice infected with the parasite N. brasiliensis. CeGFP mice carry an IRES-GFP KI cassette in the 3'UTR of membrane IgE. In these mice, GFP expression marks IgE cells, and a population of IgG1 cells with a rearrangement to Cepsilon in the non-productive (VDJ negative) IgH chromosome.

2013-10-31 | E-GEOD-49033 | biostudies-arrayexpress

Differential gene expression of human natural killer cells following immobilized IgG1 stimulation

Project description:Analysis of human natural killer cells following stimulation with immobilized IgG1 for 4 and 24 hours. Results identified significantly differentially expressed genes in natural killer cells stimulated with immobilized IgG1 providing insight into how antibodies modulate the transcriptome in way that may contribute to cell activation and immune tolerance

2019-01-24 | GSE109573 | GEO

Expression data from liver of Recombinant human IgG1 treated high-fat-diet-induced ApoE-/- mice

Project description:On the basis of the clear role of Recombinant human IgG1 in the reduction of liver triglyceride (TG) level elevated by high-fat-diet, the potential effector genes of Recombinant human IgG1 are screened by means of transcriptional techniques.

2022-12-12 | GSE190421 | GEO

Transcriptomics of BCR-stimulated IgG1 and IgG4 human B cell clones

Project description:We characterize the transcriptomic profile of a set of human IgG1 and IgG4-switched memory B cell clones

2020-05-26 | GSE129616 | GEO

Transcriptomic analysis of Human Umbilical Vein Endothelial Cells (HUVEC) in response to compound treatment

Project description:Human Umbilical Vein Endothelial Cells were treated with three newly synthesized compounds and DMSO as vehicle. Total RNA was isolated 6 and 24h after treatment and gene expression analysis was performed. Three independent experiments were performed, corresponding to rep1, rep2 and rep3. Experiment 1 (rep1) contained all substances at both time points tested. Experiment 2 (rep2) contained two of the compounds and control DMSO at both time points. Experiment 3 (rep3) contained the third compound and control DMSO at both time points.

2016-06-30 | GSE75588 | GEO

CD23+IgG1+ memory B cells are poised to switch to pathogenic IgE production in food allergy

Project description:Food allergy is caused by allergen-specific IgE but little is known about the B cell memory of persistent responses. Here we describe in pediatric peanut allergy a population of CD23+IgG1 memory B cells that contains peanut-specific clones and generates IgE plasma cells on activation. Through single cell transcriptomics and B cell receptor (BCR) sequencing, we characterized FCER2/CD23+ IgG1 memory B cells co-expressing IL4R, IL13RA1, IGHE and carrying highly mutated BCRs. Further we found that peanut allergen (Ara h 2)-specific B cells were mostly IgG1 memory cells, carried highly mutated BCRs compared to diphtheria toxin-specific B cells, and expressed FCER2 and germlin IGHE. Our findings suggest that CD23+IgG1+ memory B cells transcribing IGHE are a unique memory population containing precursors for pathogenic IgE in food allergy.

2023-11-16 | GSE208235 | GEO

IgG1 Immune Complexes Inhibit Naïve T Cell Proliferation but Stimulate a Subset of Their Progeny

Project description:We examined the effects of well-defined, soluble IgG1 immune complexes on the phenotypes of human peripheral naive CD8+ T cells. We demonstrate that IgG1-ICs inhibit the proliferation and differentiation of one subset of naïve T cells but stimulate the division of another naïve-like subset. We utilize RNA-Seq to characterize the inhibited and stimulated T cell subsets.

2021-06-26 | GSE166445 | GEO

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