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Expression data from Rhesus immune cells responding to YFV

ABSTRACT: Immune response to infection involves the regulation of numerouse genes in numerous cell types. The number and type of genes that become differentially expressed in response to infection can result in very different pathophysiologic presentations and disease course. Wild type YFV and 17D despite having very few genetic differences result in very different disease outcomes in human and monkey hosts. We used microarrays to detail the global programme of gene expression of Rhesus macaque immune cells responding to both wild type and 17D strains of YFV. We identified distinct classes of gene expression as well as marked differences in differential gene expression between responses to 17D and wild type strains of YFV.

ORGANISM(S): Macaca mulatta

PROVIDER: GSE51972 | GEO | 2014/10/22

SECONDARY ACCESSION(S): PRJNA225952

REPOSITORIES: GEO

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Expression data from Rhesus immune cells responding to YFV

Project description:Immune response to infection involves the regulation of numerouse genes in numerous cell types. The number and type of genes that become differentially expressed in response to infection can result in very different pathophysiologic presentations and disease course. Wild type YFV and 17D despite having very few genetic differences result in very different disease outcomes in human and monkey hosts. We used microarrays to detail the global programme of gene expression of Rhesus macaque immune cells responding to both wild type and 17D strains of YFV. We identified distinct classes of gene expression as well as marked differences in differential gene expression between responses to 17D and wild type strains of YFV. Rhesus PBMC were isolated before and on day 3 of either vaccination with 17D or infection with wild type YFV with an n of 3 per group. Total RNA was extracted and hybridized on Affymetrix microarrays.

2014-10-22 | E-GEOD-51972 | biostudies-arrayexpress

Gene expression profiling of PBMC from coinfected or non-coinfected mice at d0, d3, d7, and d21 post YFV-17D challenge

Project description:We tested the effects of co-infection on vaccine response to YFV-17D. Groups of mice were divided into either Mock infected or Co-infected groups. Mock infected were housed in a biohazard facility and inoculated with PBS. Co-infected were infected sequentially with murine gammaherpesvirus-68, murine cytomegalovirus, influenza WSN, and Heligmosimoides polygyrus. Both mock and co-infected groups were challenged with Yellow Fever virus (YFV-17D). Day 0 is prior to YFV. Days 3, 7, and 21 were timepoints after YFV.

2016-03-22 | E-GEOD-79466 | biostudies-arrayexpress

Gene expression profiling of PBMC from coinfected or non-coinfected mice at d0, d3, d7, and d21 post YFV-17D challenge

Project description:We tested the effects of co-infection on vaccine response to YFV-17D.

2016-03-22 | GSE79466 | GEO

ATAC-seq analysis of human CD8 T cells specific for the NS4B-214 epitope in the Yellow fever vaccine YFV-17D strain

Project description:ATAC-seq analysis of human CD8 T cells specific for the NS4B-214 epitope in the Yellow fever vaccine YFV-17D strain

2017-12-13 | GSE101609 | GEO

Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses (Experiment 2: scRNA-seq)

Project description:We performed an in-depth characterization and comparison of the immune system diversity and complexity in the spleen of conventional NRG-HIS mice and NFA2-HIS/Ftl3LG mice upon YFV-17D infection, a live-attenuated virus that induce that induce potent protective immunity in human. To do so, we employed Seq-Well, a recently developed platform for massively parallel single-cell RNA-Seq (scRNA-Seq), on splenocytes from NRG-HIS mice and NFA2-HIS/Flt3LG mice at six-weeks post YFV-17D infection. Our data provide an in-depth view of the cellular composition of the HIS in conventional and second-generation humanized mice. They also highlight the enhanced engraftment and functionality of the critical role of the myeloid and NK cell compartment in NFA2-HIS/Flt3LG mice, which is likely critical in promoting an enhanced transcriptomic, cellular and humoral response to YFV-17D.

2018-11-28 | GSE119750 | GEO

Innate signature induced in human mDCs by dengue vaccine candidates

Project description:Sanofi Pasteur is developing a tetravalent YFV 17D vaccine-based chimeric dengue vaccine (TV CYD), which is currently in clinical phase 3 development. DNA microarrays were used to assess the innate gene signature in human mDCs infected with the four CYDs alone or in combination, with a wild type dengue serotype 3 virus, or with a classically attenuated serotype 3 virus (VDV3) shown to be reactogenic in humans. We observed a very reproducible signature for each of the 4 CYDs, involving stimulation of Type I IFN genes and associated genes, together with genes encoding chemokines and other mediators involved in the initiation of adaptive responses. In contrast, the wt DEN3 virus induced a predominantly inflammatory profile, while VDV3 appeared to induce a blunted response, which may have contributed to its clinical outcome.

2011-01-03 | GSE22329 | GEO

RNAseq analysis of BDAA treatment on YFV infected HEK293 cells

Project description:Purpose: to investigate the change in gene expression after BDAA treatment on YFV infected HEK293 cells. Methods: HEK293 cells were cultured; then cells were non-infected/infected with YFV, and then untreat/treat with BDAA. Total RNA were harvested and sent to RNA sequencing. Results: we demonstrated that BDAA treatment of YFV infected HEK293 cells at 18 hpi for 2 h increased expression of a total of 39 cellular genes, 33 of which are inflammatory cytokines, chemokines or ISGs, and the remaining are mostly genes related to NFκB, TNF-α and MAPK signal transduction. Conclusion: BDAA triggers an enhanced activation of RNA sensor-mediated inflammatory cytokine responses, most likely by YFV RNA.

2021-03-04 | GSE168183 | GEO

Innate signature induced in human mDCs by dengue vaccine candidates

2011-01-03 | E-GEOD-22329 | biostudies-arrayexpress

Chromatin accessibility analysis of Huh7 cells upon YFV capsid expression

Project description:ATACseq analysis of chromatin accessibility in Huh7 cells upon YFV capsid expression

2020-11-15 | GSE152623 | GEO

YFV Capsid changes to mRNA transcriptome of Huh7 cells

Project description:Genome-wide analysis of changes to mRNA transcript levels upon expression of YFV capsid in Huh7 cells

2020-11-15 | GSE152588 | GEO