Genomic data from pooled root and shoot tissue of Arabidopsis thaliana, Arabidopsis halleri and Arabidopsis lyrata
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis halleri Arabidopsis thaliana Arabidopsis lyrata
PROVIDER: GSE52003 | GEO | 2016/09/30
SECONDARY ACCESSION(S): PRJNA226090
REPOSITORIES: GEO
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Project description:The model Zn hyperaccumulators Arabidopsis halleri and Noccaea caerulescens share elevated nicotianamine synthase (NAS) expression relative to non-accumulators among a core of alterations in metal homeostasis gene expression. Suppression of AhNAS2 expression by RNAi resulted in strongly reduced root NA accumulation and a concomitant decrease in root-to-shoot-translocation of Zn but not of other micronutrients. Few secondary effects on the root transcriptome were detected by microarray analysis. Vegetatively propagated clones of A. halleri wildtype plants and a line with strong suppression of AhNAS2 were grown hydroponically for weeks in two different media (control Hoagland and 10 µM Zn2+ added). Roots were harvested and analyzed. Three plants were pooled for one sample. Three independent experiments were performed.
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