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Genome-wide profiling of the functional DNA methylation landscape at base-pair resolution in human cancer types [Methylation BeadChip]

ABSTRACT: High-resolution DNA methylation array analysis of human cancer samples and normal control tissue types

ORGANISM(S): Homo sapiens

PROVIDER: GSE52270 | GEO | 2016/01/08

SECONDARY ACCESSION(S): PRJNA230573

REPOSITORIES: GEO

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Genome-wide profiling of the functional DNA methylation landscape at base-pair resolution in human cancer types [Methylation BeadChip]

Project description:High-resolution DNA methylation array analysis of human cancer samples and normal control tissue types Analisys of Differential Methylation Regions (DMRs) of human cancer samples and normal control tissue types performing high-resolution DNA methylation array analysis

2016-01-08 | E-GEOD-52270 | biostudies-arrayexpress

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Genome-wide profiling of the functional DNA methylation landscape at base-pair resolution in human cancer types

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Project description:We have combined standard micrococcal (MNase) digestion of nuclei with a modified protocol for construction paired-end DNA sequencing libraries to map both nucleosomes and subnucleosome-sized particles at single base-pair resolution throughout the budding yeast genome. We found that partially unwrapped nucleosomes and subnucleosome-sized particles can occupy the same position within a cell population, suggesting dynamic behavior. By varying the time of MNase digestion, we have been able to observe changes that reflect differential sensitivity of particles, including eviction of nucleosomes. Our protocol and mapping method provide a general strategy for characterizing full epigenomes. We used micrococcal nuclease mapping, chromatin immunoprecipitation and paired-end sequencing to determine the structure of yeast centromeres at single base-pair resolution.

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