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Differential expression of mature miRNAs in P19 cells during neuronal differentiation and by Rbfox3 depletion.

ABSTRACT: To investigate whether Rbfox3 could alter the expression level of miRNAs during neuronal differentiation of P19 cells, we performed miRNA microarray analysis using the RNAs extracted from untreated (undifferentiated) P19-GFP, RA-treated (neuronally differentiated) P19-GFP, or RA-treated P19-T2 cells.

ORGANISM(S): synthetic construct Mus musculus

PROVIDER: GSE52377 | GEO | 2014/09/21

SECONDARY ACCESSION(S): PRJNA227739

REPOSITORIES: GEO

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Differential expression of mature miRNAs in P19 cells during neuronal differentiation and by Rbfox3 depletion.

Project description:To investigate whether Rbfox3 could alter the expression level of miRNAs during neuronal differentiation of P19 cells, we performed miRNA microarray analysis using the RNAs extracted from untreated (undifferentiated) P19-GFP, RA-treated (neuronally differentiated) P19-GFP, or RA-treated P19-T2 cells. Total 9 samples were analyzed. We compared expression levels of P19-GFP (-) vs P19-GFP (+) vs P19-T2 (+) to identify miRNAs which had changes in expression levels with p < 0.01. From this miRNA list, we compared among P19-GFP (-) vs P19-GFP (+) vs P19-T2 (+) to identify the miRNAs which appeared to correlate with Rbfox3 expression.

2014-09-21 | E-GEOD-52377 | biostudies-arrayexpress

Gene expression profiling in Setx shRNA P19 cells, wild type human Setx P19 transfected cells with and without retionoic acid

2010-07-10 | GSE21724 | GEO

Gene expression profiling in Setx shRNA P19 cells, wild type human Setx P19 transfected cells with and without retionoic acid

Project description:Analysis of senataxin effect on neuronal differentiation and neurite growth in RA-treated P19 cells by modulating senataxin expression levels. Senataxin silencing does not grossly affect the gene expression profiles of P19 cells At day one total RNA from Setx shRNA P19 cells, wild type human Setx P19 transfected cells with and without retionoic acid were used to compare gene expression profiling versus P19 control cells

2010-07-10 | E-GEOD-21724 | biostudies-arrayexpress

BAF45D Cooperates with SMAD Signaling and Promotes Early Neural Differentiation in P19 Cells

Project description:We sought to explore whether BAF45D regulates PAX6 expression through cooperating with TGF-beta/SMAD signaling in RA treated P19 cells. Here we identified BAF45D is required for expression of phosphorylated SMAD3 and PAX6 induced by RA. Genome-wide analysis revealed that during RA-induced early neural differentiation, BAF45D knockdown failed to activate TGF-beta/SMAD signaling and induce expression of Stat3 and Smad7, two negative regulators of TGF-beta/SMAD signaling. Moreover, BAF45D was immunoprecipited with BRG1and phosphorylated SMAD3. In addition, Smad3 siRNA abolished RA-indueced expression of phosphorylated SMAD3, PAX6, STAT3 and SMAD7. Finally, overexpression of BAF45D directly induced expression of PAX6 and phosphorylated SMAD3.These results suggest that a novel effect of BAF45D cooperating with TGF-beta/SMAD signaling pathway on PAX6 level control, which may shed new light on neural differentiation of P19 cells.

2021-09-30 | GSE164125 | GEO

p19-WT and p19-T111BpyAla treatment of extracted small RNAs

Project description:small RNA fractions were treated with either p19-WT or T111BpyAla to evaluate the catalytic ability of p19-T111BpyAla compared to the WT protein in degrading miRNAs

2023-04-28 | GSE229272 | GEO

Peptide mapping of SRSF7_RS-GFP isoforms in murine P19 cells

Project description:Here we mapped peptides of mouse SRSF7_RS-GFP purified from P19 cells.

2020-05-27 | PXD016871 | Pride

RBFOX3/NeuN is required for hippocampal circuit balance and function

Project description:RBFOX3 mutations are linked to epilepsy and cognitive impairments, but the underlying pathophysiology of these disorders is poorly understood. Here we report replication of human symptoms in a mouse model with disrupted Rbfox3. Rbfox3 knockout mice displayed increased seizure susceptibility and decreased anxiety-related behaviors. Focusing on hippocampal phenotypes, we found Rbfox3 knockout mice showed increased expression of plasticity genes Egr4 and Arc, and the synaptic transmission and plasticity were defective in the mutant perforant pathway. The mutant dentate granules cells exhibited an increased frequency, but normal amplitude, of excitatory synaptic events, and this change was associated with an increase in the neurotransmitter release probability and dendritic spine density. Together, our results demonstrate anatomical and functional abnormality in Rbfox3 knockout mice, and may provide mechanistic insights for RBFOX3-related human brain disorders.

2016-07-26 | GSE84786 | GEO

RBFOX3/NeuN is required for hippocampal circuit balance and function

2016-07-26 | E-GEOD-84786 | biostudies-arrayexpress

RNA-Seq and Ribo-Seq of P19 WT and SRSF7-GFP overexpressing cells

Project description:We used P19 cells that overexpress GFP-tagged SRSF7 by 3.4-fold.

2020-03-02 | GSE142800 | GEO

The intracellular domain of amyloid precursor protein induces the dynamic change of the gene expression profile in P19 cells.

Project description:As previously reported (Neuroscience letters 444(2008)127-131), we established embryonic carcinoma P19 cell line expressing the intracellular domain of APP (AICD). Although neurons could be differentiated from these cell lines with RA treatment, expression of AICD gave rise to neuron-specific apoptosis. To identify the genes that are involved in this cell death, we evaluated changes of gene expressions that was induced by AICD through this process of cell death. The expression profiles of almost forty thousands transcripts were monitored by DNA microarrays. AICD-expressing P19 cell lines (AICD/P19) and control P19 cell lines (pCDNA/P19) that carry vector alone were used and cultured. To induce neural differentiation of these P19 cell lines, cells were allowed to aggregate in culture medium with 5×10-7 M all-trans-retinoic acid (RA) for 4 days (aggregated state). After aggregation, cells were replated onto cell culture grade dishes or poly-L-Lysine coated cell-disks for two days (differentiated state). RNAs were isolated at each states.

2010-07-14 | E-GEOD-22690 | biostudies-arrayexpress

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