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Direct sequencing of polyadenylation sites in fission yeast S. pombe

ABSTRACT: We sequenced the 3' ends of polyadenylated transcripts among total RNA in fission yeast wildtype and rdp1∆ cells and mapped the 3'-most nucleotide prior to polyadenylation. We find that mRNA-coding open reading frames have a low number of polyadenylation sites with most of the reads, whereas pericentromeric dg and dh repeats have a high number of polyadenylation sites throughout the transcribed region, mapping to both strands.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE52533 | GEO | 2013/12/26

SECONDARY ACCESSION(S): PRJNA229167

REPOSITORIES: GEO

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Direct sequencing of polyadenylation sites in fission yeast S. pombe

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2013-12-26 | E-GEOD-52533 | biostudies-arrayexpress

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Project description:Generation of Rdp1-independent primary siRNAs

| PRJNA229166 | ENA

Schizosaccharomyces pombe

Project description:Direct sequencing of polyadenylation sites in fission yeast S. pombe

| PRJNA229167 | ENA