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Analysis of flg22-induced transcriptional changes in mpk3, mpk4 and mpk6 mutants. - 2 weeks old seedlings compared for transcriptome after a 30min mock or 1µM flg22 treatment.

ABSTRACT: inra12-05_mut_flg_ii-FLG-MPKs ra12-05_mut_flg_ii - inra12-05_mut_flg_ii - Analysis of flg22-induced transcriptional changes in mpk3, mpk4 and mpk6 mutants. - 2 weeks old seedlings compared for transcriptome after a 30min mock or 1µM flg22 treatment.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE52587 | GEO | 2014/06/16

SECONDARY ACCESSION(S): PRJNA229415

REPOSITORIES: GEO

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Analysis of flg22-induced transcriptional changes in mpk3, mpk4 and mpk6 mutants. - 2 weeks old seedlings compared for transcriptome after a 30min mock or 1ÂµM flg22 treatment.

Project description:inra12-05_mut_flg_ii-FLG-MPKs ra12-05_mut_flg_ii - inra12-05_mut_flg_ii - Analysis of flg22-induced transcriptional changes in mpk3, mpk4 and mpk6 mutants. - 2 weeks old seedlings compared for transcriptome after a 30min mock or 1ÂµM flg22 treatment. 21 dye-swap - gene knock out,normal vs mutant comparison,treated vs untreated comparison

2014-06-16 | E-GEOD-52587 | biostudies-arrayexpress

mpk6 flg22 - Flagellin response in mpk mutants

Project description:au-09-01_mpk_flagellin_edu - mpk6 flg22 - Investigate flagellin mpk dependent genes - 2 weeks mpk6 flg22 treated 1h with 1 microM flg22. Keywords: treated vs untreated comparison

2012-01-01 | GSE23193 | GEO

mpk4 flg22 - Flagellin response in mpk mutants

Project description:au-09-01_mpk_flagellin_edu - mpk4 flg22 - Investigate flagellin mpk dependent genes - 2 weeks mpk4 plants treated or untreated with 1h with 1 microM flg22 Keywords: treated vs untreated comparison

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mpk6 flg22 - Flagellin response in mpk mutants

2012-01-01 | E-GEOD-23193 | biostudies-arrayexpress

mpk4 flg22 - Flagellin response in mpk mutants

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Expression analysis of Arabidopsis mpk11 mutant treated with flg22

Project description:Arabidopsis MPK11 is activated and plays a role in the flg22 sensing. Mitogen-activated protein kinases (MAPKs) mediate cellular signal transduction during stress responses, as well as diverse growth and developmental steps in eukaryotes. Pathogen infection or treatment with conserved pathogen-associated molecular patterns (PAMPs) such as the bacterial flagellin-derived flg22 peptide are known to activate three Arabidopsis thaliana MAPKs, MPK3, MPK4 and MPK6. Several stresses, including flg22 treatment, are known to increase MPK11 expression but activation of MPK11 has not been shown. Here, we show that MPK11 activity can indeed be increased through flg22 elicitation. Expression profiling using a small-scale microarray for defense-related genes revealed that cinnamyl alcohol dehyrogenase 5 (CAD5) requires MPK11 for full flg22-induced expression. An mpk11 mutant showed increased flg22-mediated growth inhibition but no altered susceptibility to Pseudomonas syringae, Botrytis cinerea or Alternaria brassicicola.

2012-05-03 | GSE33648 | GEO

inra12-01_mut-flg-FLG-OoPTIMICE

Project description:ra12-01_mut_flg - inra12-01_mut-flg - Analysis of flg22-induced transcriptional changes in oxi1, OXI1oe, pti1-4, CA-MPK4 and ice1 mutant lines - 2 weeks seedlings compared for their transcriptome after a 30min mock or 1µM flg22 treatment.

2014-12-01 | GSE46152 | GEO

Expression analysis of Arabidopsis mpk11 mutant treated with flg22

2012-05-02 | E-GEOD-33648 | biostudies-arrayexpress

mpk4/mpk4 gof vs mpk4/mpk4 vs col0 -Transcriptome study of mpk4 complemented line with a hyperactive form of MPK4 (MPK4 GOF).

Project description:au10-07_mpk4gof - mpk4/mpk4 gof vs mpk4/mpk4 vs col0 - Genes regulated by MPK4 pathway. - In order to study of the genes controlled by MPK4 pathway we generate mpk4 complemented lines expressing active MPK4 (MPK4 GOF). For the transcriptome analysis we compare the MPK4 GOF to mpk4/MPK4 (complemented mpk4 mutant with a wt MPK4) and to Col0. Keywords: genotype comparaison

2011-08-20 | GSE23664 | GEO

MPK4 phosphorylation dynamics and interacting proteins in plant immunity

Project description:The Arabidopsis MAP kinase 4 (MPK4) has been proposed as negative player in plant immunity, but it is activated by pathogen-associated molecular patterns (PAMPs), such as flg22. The molecular mechanisms by which MPK4 is activated and controls plant defense remain elusive. In this study, we investigated the Arabidopsis defense against a bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 when the MPK4 is overexpressed. We showed an increase in pathogen resistance and suppression of jasmonic acid (JA) signaling in the MPK4 overexpressing (OE) plants. We also showed that the OE plants are very sensitive to flg22-triggered reactive oxygen species (ROS) burst in guard cells, which resulted in an enhanced stomatal closure, compared to wild-type (WT). During the flg22 activation, dynamic phosphorylation of MPK4 within and outside of the conserved TEY activation loop was observed. To elucidate how MPK4 functions during the defense response, we used immunoprecipitation coupled with mass spectrometry (IP-MS) to identify MPK4 interacting proteins in the absence and presence of flg22. Quantitative proteomic analysis revealed a shift in MPK4-associated protein network, providing insight into the molecular functions of MPK4 at a systems level.

2019-01-14 | PXD009016 | Pride

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