Project description:Wnt-4 signaling is critical for embryonic female sexual development. When Wnt-4 gene is deleted during embryonic development, the knock-out females present a partial sex reversal. We used microarrays to detail the global programme of gene expression underlying sexual development and identified distinct classes of up and down-regulated genes during this process. Mouse embryonic gonad from wild type and Wnt-4 knock-out mice (male and female) were selected at two stages of sex determination for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of embryonic gonad at each developmental stage in order to increase the temporal resolution of expression profiles. To that end, we hand-selected embryos according to morphological criteria at two time-points: E 12.5 and E14.5.

2015-06-30 | E-GEOD-52729 | biostudies-arrayexpress

Expression data from E12.5 and E14.5 mouse embryonic gonad of wild type (WT) and Wnt-4 knock-out (KO) mice. [Mouse430_2]

2015-06-30 | E-GEOD-51089 | biostudies-arrayexpress

Global gene expression of Oscar Knock-out (KO) mice with Wild Type (WT) mice.

Project description:Purpose: RNA sequencing (RNA-Seq) analyses of articular cartilage obtained after DMM surgery in Oscar-/- and WT mice. The purpose of this experiment was to demonstrate how the deficiency of Oscar gene affects downstream signal transduction in articular cartilage. Methods: Articular cartilage mRNA profiles of 10-weeks-old wild-type (WT) and Oscar knockout (Oscar−/−) mice were performed for the destabilization of the medial meniscus (DMM) and each cartilage tissue sample was harvested from two time-points (2- and 4-weeks after surgery). Sequencing libraries were prepared according to the manufacturer’s instructions (TruSeq Stranded mRNA Library Prep Kit; Illumina, San Diego, CA, USA). Paired-end sequencing of 101-mer read length was performed using a HISEQ 2500 sequencing system (Illumina). The sequencing quality of raw FASTQ files was assessed using FastQC (https://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Low-quality reads and adapter sequences in reads were eliminated using BBDuk (http://jgi.doe.gov/data-and-tools/bb-tools/). Results: Usinig RNA-Seq data in articular cartilage tissues from WT and Oscar–/– mice subjected to sham or DMM surgery and each cartilage sample obtained from 2- and 4-week time points after surgery, respectively. And we found that significantly enriched pathways in the 1270 common genes. Conclusions: Our study represents the Oscar gene deficiency analysis of articular cartilage transcriptomes, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of mRNA content within a cell or tissue. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.

2020-03-26 | GSE147529 | GEO

Comparative testicular transcriptome of wild type and globozoospermic Dpy19l2 knock out mice.

Project description: Not available

| S-EPMC4346239 | biostudies-literature

Quantitative proteomics of nutrient starved wildtype (WT) and MTM1 knock out (KO) HeLa cells.

Project description:Quantitative whole cell proteomics that compare fed or starved wildtype (WT) and MTM1 KO HeLa cells.

2022-12-22 | PXD033850 | Pride

Microarray of wild type, Shp2 knock-out, Pten knock-out and double knock-out erythroblasts

Project description:the gene expression profiling results provide important information for the genes regulated by crosstalk between Shp2 and Pten mediated signal pathways

2013-07-03 | GSE48477 | GEO

CRISPR/Cas9-Mediated Knock-Out of dUTPase in Mice Leads to Early Embryonic Lethality.

Project description: Not available

| S-EPMC6523736 | biostudies-literature