Transcriptomics,Genomics

Dataset Information

33

Effects of rapamycin exposure on zebrafish ZF4 cells


ABSTRACT: mTOR inhibitor rapamycin is a well-known anticancer and immunosuppressant agent. Effects of rapamycin on zebrafish cells have not been previously studied using transcriptome analyses. Our microarray analysis on ZF4 cells showed that rapamycin treatment modulated a large set of genes with varying functions including protein synthesis, assembly of mitochondrial and proteosomal machinery, cell cycle, metabolism, and oxidative phosphorylation. Overall design: Zebrafish ZF4 cells exposed to 100nM rapamycin was profiled using Zebrafish Affymetrix Gene Chips. Normalization by RMA and class comparison analyses were performed using BRB Array Tools.

INSTRUMENT(S): [Zebrafish] Affymetrix Zebrafish Genome Array

SUBMITTER: Ozlen Konu  

PROVIDER: GSE53086 | GEO | 2016-12-31

SECONDARY ACCESSION(S): PRJNA230818

REPOSITORIES: GEO

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Publications

Functionally conserved effects of rapamycin exposure on zebrafish.

Sucularli Ceren C   Shehwana Huma H   Kuscu Cem C   Dungul Dilay Ciglidag DC   Ozdag Hilal H   Konu Ozlen O  

Molecular medicine reports 20160328 5


Mechanistic target of rapamycin (mTOR) is a conserved serine/threonine kinase important in cell proliferation, growth and protein translation. Rapamycin, a well‑known anti‑cancer agent and immunosuppressant drug, inhibits mTOR activity in different taxa including zebrafish. In the present study, the effect of rapamycin exposure on the transcriptome of a zebrafish fibroblast cell line, ZF4, was investigated. Microarray analysis demonstrated that rapamycin treatment modulated a large set of genes  ...[more]

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